The goal of the present study was to determine the salinity resistance of 5 triticale genotypes. Salt-stress experiments were carried out under tissue culture conditions. Regenerated seedlings of five genotypes were exposed to 0 and 100 mM NaCl. Peroxidase activity and pigment content were determined for seedling applied with salt for 7, 14, and 21 days. Peroxidase activity was observed in the triticale seedlings of salt stress in in-vitro conditions. Melez 2001 and Mikham 2002 were determined to be more responsive to stress severity than the other genotypes in terms of chlorophyll content. Our results showed that there was a positive correlation between chlorophyll content and 100 mM concentration salt stress in salt-resistant triticale genotypes.