This study investigated the antimicrobial and antibiofilm activities of Ferula species (sp.) obtained from Erzurum province. Antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Candida albicans, and Candida tropicalis were determined by agar well diffusion assay. Ethyl acetate extract showed antimicrobial activity against S. aureus, and methanol extract showed antimicrobial activity against E. coli and C. albicans with a zone diameter of 21, 18, and 15 mm, respectively. The extract's minimum inhibitory concentrations (MIC) were 16, 32, and 32 μg/mL ml for S. aureus, E. coli, and C. albicans, respectively. Furthermore, it was determined for the first time that Ferula sp. extracts were effective against biofilm formations of S. aureus and C. albicans. In the light of these results, we think that extracts of Ferula sp. contain potential candidate molecules against biofilm-associated infections, and further characterization studies are needed.