Yumurtlamayan piliçlerle yumurta tavuklarının kan serumlarında protein ve elektroforetik franksiyonları yönünden araştırmalar

Bu araştırmada sağlıklı 0-4 aylık 100 adet beyaz Leghorn dişi piliç ile ile 5~9 aylık 100 adet yumurUayan beyaz Leghorn tavuk kullanıldı. Piliç ve tavuk serumlarının total serum proteinleri Biüret yöntemi ile protein fraksiyonlan da sellüloz asetat elektroforezi tekniğiyle belirlendi . . serum total protein konsantrasyonunun yaşla artış gösterdiği tespit edildi. Serum proteinleri seUüloz asetat elektroforezi ile, O yaşta anattan katoda doğru hareket yeteneği azalan 5 farklı bant, 1 - 7- aylık dişi piliç ve· yumurtlayan tavuklarda 7 farklı bant, 8 ve 9 aylık yumurtlayan tavuklarda 6 farklı bant gözlemlendi. Elektroforezde total serum prot~İll konsantrasyonunun en büyük kısmını o yaşta gammaglobulin'in, 1 - 5 aylıklarda albumin'in, 6-9 aylıklarda ise gammaı-globulin'in teşkil ettiği bulundu. Yaşın ve yumurtlamanın serum total proteinin ve fraksiyonlannın üzerinde değiştirici bir etki yaptığı belirlendi. Albumin fraksiyonunun yaşla yükseldigi fakat bu yükselişin tavuklann yumurtlama peryoduna kadar hızlı ve yumurtaya geçtikten sonra düştüğü, globülin fraksiyonunun ise 4 aylığa kadar düşüş gösterdiği daha sonra yükseldiği saptandı. A/G oranının 2. ve 3. aylarda en yüksek (0 .97) değerde olduğu, daha sonraki aylarda ise yaşla tedricen düşüş gösterdiği izlendi. · 0 - 7 aylık dişi piliç ve yumurtlayan tavuklarda prealbumin bandı tek olarak tespit edilmesine karşılık 8 ve 9 aylık yumurtlayan tavuklarda varlığı gösterilemedi.

Anahtar Kelimeler:

Yumurtlamayan Pliç, Yumurta Tavukları, Kan Serumlarında Protein, Kan Serumlarında Elektroforetik Fraksiyon, Necla ERKAL, 1979, 1980, 1981

Studies on the serum protein and its electrophoretic fractions in laying and non laying hens.

During this study 0-4 months old ıoo White female healthy Leghorn chicken and 5-9 months old ıoo White Leghorn laying hens were used. Total serum protein of chicken and hen sera were determined by Biüret method, protein fractions were determined by cellulose- acetate electrophoresis. 1 Serum total protein consentration was increasing by age. Serum proteins of o age by cellulose - acetate electrophoresis showed 5 different bands which moving ability were decreasing from anode to cathode. In the· serum proteins of 1-7 months old female chicken and laying hens 7 different bands were observed and their movement also were decreasing from anode to cathode. In the serum proteins of 8 and 9 months old laying hens 6 different bands were determined and their mavement also were decreasing from anode to cathode. In electrophore.sis, gammaı-globulin was the highest fract ion in total serum protein consentration of o age, albumin was the highest in 1-5 months and gammaı-globulin was the highest in 6-9 months laying hens. The transformar effects of the age and the laying factors were investigated on the serum total protein and its fractions. The fraction of albumin was increasing by age but this increas was fast till to laying period and decreasing after that. Also globulin fraction was decreasing till 4 months and after that begin to increas.The ratio of A/G was the highest at 2nd and 3th months {0.97) and then gradually decrease by age. Prealbumin was obse·rved as one band in 0-7 months but couldn't determined in 8 and 9 months laying hens.

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