Sığırlarda Theileria Türlerinin Reverse Lıne Blottıng Ve İndirek Floresan Antikor Testi İle Karşılaştırmalı Tanısı Üzerine Araştırmalar

Bu çalışma, 2001-2002 tarihleri arasında Ankara bölgesinde RLB(Reverse Line Blotting), IFA (Indirek Floresan Antikor testi)testleri ve mikroskobik bakı ile sığırlarda Theileria türlerinin tanısını yapmak, tanı yöntemlerinin karşılaştırmalı geçerliliğini tespit etmek, ayrıca eşzamanlı, yani bir örnekle aynı soya bağlı birden fazla türün saptanabildiği RLB testinin rutin olarak laboratvarlarda ve özellikle epidemiyolojik çalışmalarda kullanılabilirliğini ortaya koymak amacıyla yapılmıştır. Bu amaçla, Ankara İline bağlı 9 ilçeye ait köylerde meraya çıkan hayvanlardan, rastgele seçilen 250 sığırdan IFAT ve RLB testlerinde kullanılmak üzere kan alınmıştır. Sonuç olarak, mikrsokobik bakıda %29,6; IFA testinde (t. annulata) %28.8; RLB yöntemlerinde ise %47,6 oranında pozitiflik tespit edilmiştir. Kullanılan yöntemler duyarlılık ve özgüllük bakımından karşılaştırıldığında RLB'ye göre mikroskobik bakının %59,8; IFAT (T.annulata)ın ise %64,6 oranında duyarlı olduğu görülmüştür. Bu çalışma ile Türkiye'de ilk defa sığırlarda Theileria buffeli tespit edilmiştir.

Anahtar Kelimeler:

Ankara, Theileria annulata, Theileria buffeli, IFAT, RLB, Sığır, 2006, Etlik Veteriner Mikrobiyoloji Dergisi, Ahmet DENİZ, Zafer KARAER

"Comparative Studies on Oetection of Bovine Theileria Species by Reverse Line Blotting and Indirect Fluorescent Antibody Test"

This study was carried out to diagnose Theileria species in cattle by using, RLB, IFA and microscopic examination; to compare the diagnosis methods; in addition to detect the value of the usage of RLB, that can detect more species belong to the same family, routinliy in laboratory and particularly in epidemiological studies in Ankara region between 2001-2002. For this purpose the blood samples were randomly collected from 250 cattle from the village of 9 towns of Ankara province. At the end of the examination of the samples according to the tests, 29,6%positivity was detected by microscopic examination, 28,8% by IFA and 47,6 by RLB. When the tests used were compared according to RLB regarding to their sensitivity and specifity, th esensitivity of microscopic examination was detected to be 59,8% and IFA 64,6%. The differences among the three tests were statistically found to b significant and it is cancluded that RLB was more sensitiv and specific compared to the other two tests. By this study, Theileria buffeli was firstly detected in cattle in Turkey.

Keywords:

Ankara, Theileria annulata,

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