Özden KABAKLI, İbrahim HANCI, Süreyya YÖNDEM

Liyofilize Attenue Koyun- Keçi Vebası Aşısı Raf Ömrünün Belirlenmesi

Bu çalışma Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü tarafından üretilen liyofilize attenue PPR (koyun- keçi vebası) aşısının -20°C ve +4 (+2/+8) oc dereceler arasındaki raf ömrünün saptanması amacıyla yapıldı. Araştırmada Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü tarafından üretilen PPR 0277-4, PPR 0277-4A, PPR 0377-5 seri nolu aşıların raf ömürleri 24 ay süreyle kontrol edildi. Aşıların titreleri liyofilizasyon tarihlerinde PPR 0277-4 ve PPR 0277-4A seri nolu aşılarda ıo-6.4 - ıo-6 ·3 DKID50/ml olarak bulunur iken +4 (+2/+8) oc derecede depolanan aşılarda 24. ayda titrenin ıo-6 · DKID50/ml'e düştüğü , PPR 0377-5 seri nolu aşılarda 1 o-6 8 DKID50/ml olarak bulunur iken 24. ayda titrenin ıo-6 ·5 DKID50/ml'e düştüğü saptandı fakat bu düşüş istatistiki açıdan önemli bulun- madı. Aşıların -20°C de bekletilmesiyle titrelerinde değişiklik olmadığı belirlendi. Bu çalışma ile liyofilize attenue PPR (koyun-keçi vebası) aşısının -20°C ve +4 ( + 2/+8)°C dereceler arasındaki raf ömrünün 2 yıla (24 aya) çıkarılmasının uygun olacağı sonucuna varılmıştır.

Anahtar Kelimeler:

Koyun, Keçi, Veba, Aşı, Özden KABAKLI, ibrahim HANCI, Süreyya YÖNDEM, 2006, Etlik Veteriner Mikrobiyoloji Dergisi, Koyun Keçi Veba Aşısı

"Determination of shelf- life of Peste des petits ruminants vaccine"

The aim of this study w as to determine the selflife of lyophilized attenue PPR vaccine (Sheep and Go at Plague) produced by Etlik Central Veterinary Control and Research Institute at between -20 °C and +4 (+2/+8) oc. At the study PPR 0277-4, PPR 0277-4A and PPR 0377-5 seri- al numbered vaccines had been controlled for 24 months. Whereas the titres of PPR 0277-4 and PPR 0277-4A serial numbered vaccines were deter mined as 1 o-6.4 - 1 o-6· 3 TCID50/ml at the lyophilized time, it was noticed that the titres were decreased to ıo-6· ı TCID50/ml after 24 months storage period at +4 (+2/+8) C. While the titre ofPPR 0377-5 serial numbered vaccines show ed 1 o-6· 8 TCID50/ml at the lyophilized time, it was decreased to 1 o-6 ·5 TCID50/ml after 24 months but it was found no significant statistically. It was noticed that there was no changes at the titres of the vaccines with storing them at -20 oc . According to the result of this study it will be can venient to extend the shelf-life of the PPR vaccine up to 24 months at -20 oc ve +4 (+2/+8) oc

Keywords:

PPR, Vaccine, Shelf-life,

PDF

___

ANONiM. : Peste des petits ruminants. In;OIE Manual of Standarts Diagnostic Tests and Vaccines. 5th Ed. Chapter 2. ı .5. (2004)
BAILEY,N.T.J.; Statical Methods in biology. Second Edition. A division ofHodder&Stoughton, London, ı 98 ı.
BERHE, G., MINET, C., LE GOFF, C., BARRETT, T.~ NGANGNOU, A., GRILLET, C., LIBEAU, G., FLEMING, M., BLACK, D.N., DIALLO, A: Development of a dual recombinant vaccine to protect small ruminants virus and capripoxvirus infections. J. Viral. Jan;77(2):ı57ı-ı577. (2003)
CAUACY- HYMANN, E., BIDJEH, K., ANGBA, A., DOMONECH, J., DIALLO,A.: Protection of goats against rinderpest by vaccination with attenuated peste des petits ruminants. Res. Vet.Sci., 59: ıo6-ı09. (1995)
DIALLO, A.: Rinderpest and peste des petits ruminants; constant threats to animal farming in many developing countries. Impact Sci. Society. 150: 179- ı 92. (1988)
DIALLO,A.: Control of peste des petits rumi- nants: classical and new generatian vaccines. Dev. Biol.( Basel). ı14: ıB - ı19. (2003b)
DIALLO, A., TAYLOR, W.P., LEFEVRE, P.C., PROVOST, A.: Attenuation d' une souch de virus de la peste des petits ruminants: candidant pour un vaccin homoloque vivant. Revue. Elev. Mad. Vet. Pays.Trop. 42 (3): 3ıı-3ı9 . (1989)
GÜLYAZ ,V.: Bakırköy koyun-keçi çiçek aşının raf ömrünün saptanması. Pendik Veteriner Mikrob. Der. 34(1-2): 11-1 7.(2003).
JONES, L., GIAVEDONI, L., SALIKI, J.T., BROWN, C., MEBUS, C., YILMA, T.: Protection of goats against peste des petits ruminants with a vacCiriia virus double recombinant expressing the F and H genes of rinderpest virus. Vaccine, ı ı(9) : 96ı-964 . (1993)
MARTRENCHAR,A., ZOYEM. N., DIALLO, A. : Experimental study of a mixed vaccine against peste des petits ruminants and capripox infection in goats in northern Cameroon. Smail Ruminants Research, 26: 39-44. (1997)
MARTRENCHAR, A., ZOYEM. N., NJOYA, A., NGO TAMAA.C., BOUCHEL, D., DIALLO, A.: A field study of an homologous vaccine against peste des petits ruminan ts in northern Cameroon. Small Ruminants Research.3ı(3). (ı999)
PLOWRIGHT W.,HERNIMAN K.A.J,RAMPTON C.S., TAYLOR W.P.: Studies on Rinderpest Culture Vaccine. III. Stability of The Lyophilised Product. Res. Vet. Sci. ll: 7ı-8ı , 1970.
PLOWRIGHT W., HERNIMAN K.A.J, RAMPTON C.S. : Studies on Rinderpest Culture Vaccine. IV.The Stability of Reconstituted Product. Res. Vet. Sci. ı2 : 40-46, ı971 .
SARKAR, J., SREENIVASA, BP.,: Comparative efficacy ofvarious chemical stabiliz- ers on the thermostability of a live - attenuated peste des petits ruminants( PPR) vaccine. Vaccine ;21 (32): 4728-35, 2003
SCOTT,G.R. : Peste des petits ruminants (Goat plaque). In: Virus infections of Ruminants. Edd: Z.Dinter, B., Morein, Elseveir Science Publisher, Amsterdam, Oxford, Newyork, Tokyo, chapter, 32-33p.: 34ı-361. (1990)
TAYLOR, W.P. : The distribution and epidemiology of peste des petits ruminants. In: Impact of diseases on Livestock Production in the Tropics. Edd: H.P. Reimann, M.J. Burridge, Elsevier, p: ı 57-166. (ı 984)
VALLY,K.J.M., AHBA, K., SHAILA, M.S., LAKSHMI SITA, G. : Development of transgenic plants of edible vaccine for peste des petits ruminants, an animal disease. In Vitro Cell. Dev. Bi ol. 38: ı 171. (2002)
WOSU,L.O.: Current status ofpeste des petits ruminants (PPR) disease in small ruminants a review article. Stud. Res. Vet. Med 2: 83-90. (1994)