Hakan YARDIMCI, Ömer ESENDAL, Nejat AYDIN

Sığır Brucellosis'inin Serum Aglutinasyon, Komplement Fikzasyon ve İmmunocomb Testleriyle Teşhisi

Bu çalışmada, sığırlarda brucella infeksiyonuna karşı oluşan spesifik antikodann ortaya konulmasında rutin olarak kullanılan Serum Aglutinasyon (SAT) ve Komplement Fikzasyon ( CFT) testlerinin yanısıra enzim immunoassay prensibi ile çalışan ticari immunocomb test (IC) kitinin rutin uygulamaya dahil edilip edilerniyeceği araştırıldı. Abort yapmış veya bruceila şüpheli 68 sığırın kan serumlan SAT, CFT ve lC testleri ile değerlendirildi . Sırasıyla, 38 (%56), 28 (%41) ve 38 (%56) pozitif serum belirlendi. Buna göre, pozitifleri belirlemede IC=SAT>CFT sırası elde edildi. Sığır brucellosis 'nin serolojik teşhisinde, değişik antikor gruplan (lgM,IgG) ile çalışan konvansiyonel testler (SAT,CFT,vs) yanısıra immunocomb gibi duyarlı, pratik, çabuk ve saha koşullannda da yararlanılabilen bir testin de kullanılmasının, infeksiyenun teşhisinde yararlı olacağı sonucuna vanldı.

Anahtar Kelimeler:

Sığır, Brucellos, Serum, Aglutinasyon, Komplement Fiksasyon, İmmunocomb, Sığır Brucellosis'in Teşhisi, Hakan YARDIMCI, Ömer ESENDAL, Nejat AYDIN, 1996, Etlik Veteriner Mikrobiyoloji Dergisi, cft

The Diagnosis of Bovine Brucellosis With Serum Agglutination, Complement Fixation and İmmunacob Tests

In this study, the use, efficacy and reliability of the commerical ImmunoCom b test kit, which works according to the enzyme immunoassay principle, together with other serological tests such as SAT and CFT in the detection of specific antibodies against bovine brucellesis were investigated. A total of 68 bovine sera obtained from brucellesis suspected as well as aborted cattle were examined with SAT,CFT and IC.38 (56%), 28 (41 %) and 38 (56%) sera were proved to be positive, respectively. Therefore, IC=SAT>CFT . result was obtained in the detection of specific antibodies. As a result, the use of İC, which is a sensitive, practical and rapid method, together with other conventional tests working with different antibody groups (IgM,IgG) such as SAT and CFT, was proved to be beneficial in the diagnosis of the infection.

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