Sedat SEVİN, Mehmet Eray ALÇIĞIR, Mustafa ÖZKURT, Zekeriya YURDABAKAN, Ömer YALÇIN, Ender YARSAN

Ratlarda Scillaren-A, mısır koçanı tozu ve kombinasyonları ile ilişkili akut toksisitelerin karşılaştırmalı histopatolojik değerlendirilmesi

Çalışmanın amacı Scillaren-A’nın ratlarda etkinliğini karşılaştırmalı olarak değerlendirmekti. Kırk sekiz saat boyunca akut toksisite geliştirmk için toplam 32 dişi Wistar albino rat kullanıldı. Ratlar rastgele dört gruba ayrıldı (her grupta n=8 / G.I-G.IV). Toksisite grupları (G.I, G.II ve G.III) ve kontrol grubu (G.IV) oluşturuldu. Deney grupları sırasıyla scillaren A, mısır koçanı tozu ve bunların kombinasyonu ile zehirlendi. Kontrol grubundaki ratlar zehirlenmedi ve standart peletler ile beslendi. Toksikasyondan 48 saat sonra deney sonlandırıldı. Yaşamsal organlarda (karaciğer, böbrek, akciğer, kalp, beyin) ve ardından diğer organlarda (bağırsak, dalak) histopatolojik bulgular değerlendirildi. Dokular %10’luk tamponlu formalin (pH=7.2) içinde tespit edildi ve parafine gömüldü. Histopatolojik bulgular standart H&E boyalı kesitlerde değerlendirildi. Organlardaki önemli histopatolojik değişiklikler, hiperemi ve hemorajiyi içeren vasküler değişiklikler, dejenerasyon ve nekrozu içeren alteratif değişiklikler, yangı, hiperplazi ve fibrozis gibi bazı rejeneratif ve reperatif değişiklikler skorlandı. Her iki formülasyonun da ilerleyen zamanda ölüm gelişmesinde etkili olabileceğine, çevresel toksik etkisi olmaksızın bir rodentisit olarak kullanılabileceğine ve çekici kimyasal özellikleri nedeniyle ratlar tarafından hızla tüketilebileceğine inanılmaktadır.

Anahtar Kelimeler:

Histopatoloji, mısır koçanı tozu, rat, Scillaren-A, toksikasyon

A comparative histopathological evaluation of acute toxicities associated with Scillaren-A, corn cob powder and their combinations in rats

The purpose of the study was to comparatively evaluate the efficacy of Scillaren-A in rats. A total of 32 female Wistar albino rats were used to develop acute toxicity for 48 h. The rats were randomly divided into four groups including n=8 / each group (G.I-G.IV). Toxicity groups (G.I, G.II, and G.III) and control group (G.IV) were established. The experimental groups were intoxicated with scillaren A, corn cob powder, and their combinations, respectively. The rats in the control group were remained intoxicated and fed with standard pellets. The experiment was ended 48 hours after the intoxication. Histopathological findings in vital organs (liver, kidney, lung, heart, brain) and then other organs (intestine, spleen) were evaluated. Tissues were fixed in 10% buffered formalin (pH=7.2) and embedded in paraffin. The histopathologic findings were evaluated on the standard H&E stained sections. The major histopathological changes, vascular changes including hyperemia and haemorrhagia, alterative changes including degeneration and necrosis, inflammation, some regenerative and reparative changes including hyperplasia and fibrosis in organs were scored. It is believed that both formulations can be effective on death later on, be utilized as a rodenticide without environmental toxic effect, and be consumed rapidly by rats due to their attractive chemical traits.

Keywords:

Corn cob powder, histopathology, intoxication, rat, Scillaren-A,

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