Monoklanal Antikorların Newcastle Hastalığının Teşhis ve Epidemiyolojisinde Kullanımı

Poliklonal antikorların konvansiyonel serolajik testlerde virusların ayrımında kullanılması bazı problemlere yol açmaktadır. Monoklonal antikor teknolojisi (mAb) konu için yeni bir alternatif olmuştur. Newcastle Hastalığı Viruslarına (NHVJ karşı hazırlanan mAb'ler değişik izolatlar arasındaki kayda değer antijenik farklılığı göstermişlerdir. NHV suşlarının hepsi yada pek çoğu için spesifik olan ve HI aktivitesi gösteren mAb'ler. Newcastle Hastalığı (NH) için referans laboratuvar olan Weybridge'de rutin teşhiste kullanılmaktadır. Bu mAb'lerin kullanımı NHV. variant NHV. Lasota ve PMV-3 arasında ayrımı sağlamaktadır. MAb'ler aynı zamanda NHV'larının antijenik yapılarına göre gruplanmalarını sağlamışlardır. Bu konudaki en başarılı yaklaşım Russell ve Alexander ' ın çalışmaları olmuştur. Araştırıcılar, NHV'larını, NHV Ulster 2C suşuna karşı oluşturulan 9 mAb ile reaksiyonlarını temel alarak gruplamışlardır. Oluşturulan grupların virulans, temel konakçı gibi epidemiyolojikal özellikleri büyük ölçüde paylaştığı görülmüştür. MAb gruplamasının NH'nin epidemiyolojisindeki başarısı. İngiltere'de variant NHV'larının tavuklarda oluşturduğu NH salgınlarını ve Türkiye'de Güney Marmara bölgesinde son zamanlarda görülen NH salgınını da içeren pek çok olguda kanıtlanmıştır.

Anahtar Kelimeler:

Etlik Veteriner Mikrobiyoloji Dergisi, Newcastle, Monoklonal Antikorlar, Epidemiyoloji, 1999

Use of Monoclonal Antibiotics in Diagnosis And Epidemiology or Newcastle Disease

Applications of monoclonal an tibody (mAb) technology for Virus characterisation has been an alternative approach since polyclonal antibodies have been shown to have some problemsin discriminating Viruses in conventional serological tests . MAbs to Newcastle disease virus (NDVJ have proved the considerable variation among different isola tes. Many laboratories have prepared mAbs to NDVs and shown conserved and variant epitopes on some isolates. MAbs that is spesific for all or vast majority of NDV strains and show HI actiVities are now being used in Weybridge. international referans laboratory for Newcaslle Disease (ND), for routine diagnosis. Use of these mAb's enables distinction between NDV, variant NDV. Lasota and PMV-3 Viruses. MAbs also served to group NDVs with regard to their antigenic properties. The most successful approaches on this subject were studies of Russell and Alexander. They grouped NDV strains on the hasis of their reaction with 9 mAbs to NDV Ulster 2C. The groups formed have shared im portant epidemiological properties, such as main host, virulance ete. Success of mAb grouping in epidemiology of the ND has been confirmed in many ND casesincluding ND outbreakin chickens due to variant NDVs in England and recent ND outbreakin south Marmara region of Turkey.

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ABENES G, KIDA H, YANAGAWA R, (1986). Antijenilc mapping andjunctional analysis oj the F protein oj Newcastle Disease virus using Monocianal Antibodies. Arch. Virol. 90 97-110
ALEXANDER DJ, (1980). Avian Paramyxovirusses VEt. Bull. 50: 537-572
ALEXANDER DJ, (1985). Antijenic and biological characterization oj Avian Paramyxoirusses. Type-1 isolatesfrom pigeons-an international collabarative study. Avian Pathol. 14:365-376
ALEXANDER DJ, (1990). Avian Paramyxoviridae-Recent developments. Vet. Mic. 23. 103-114
ALEXANDER DJ, (1991). Newcastle Disease and other paramyxovirus injections. Diseases ofpoultry, 9. Edition, Iowa state university press lowa. 1101-1108.
ALEXANDER DJ, (1992). Use oj monocianal antibodies in the diagnosis oj Newcastle disease and characterization oj the viruses. W arkshop on Avian Paramyxoviruses. Edition, Commission of the European communities 145-156
ALEXANDER DJ, (1995). Neewcastle Disease in countries oj the European Union. Avian Pathol. 24 3-10
ALEXANDER DJ, PARSONS G, MARSHALL R, (1984). Injection oj jowls with Newcastle disease virus by jood contaminated with pigeonjaeces Vet. Rec. 115: 601-602
ALEXANDER D J, WILSON GW C, RUSSEL P H, LISTER S A, PARSONS G, (1985). Newcastle Disease outbreaks injowl in Great Britain during 1984 Vet. Rec. 117: 429-434
ALEXANDER D J, MACKENZIE J S, RUSSELL P H, (1986). Two types oj Newcastle disease virusses isolatedjromjeral birds in Western Australia detected by monocianal antibodies. Aust. Vet. J. 63: 365-367
ALEXANDER DJ, MANVELL RJ, KEMP PA, PARSON S G, COLLYNS M S, BRACK MAN S, RUSSELL PH, LISTER SA. ( 1987). Use oj monocianal antibodies in the characterization oj Av ian paramyxovirusses type 1 isolates submitted to an iriternational rejerence laboratory. Avian Pathol. 16: 553-565
ALEXANDER DJ, MANVELL RJ, COLLINS M S, BROCKMAN S J, (1991). Evaluation oj relationships between Avian paramyxovirusses isolatedjrom birds oj the family Columbidae. Arch. Virol 116: 267-276
ALEXANDER D J, CAMPBELL G, MANVELL R J, COLLINS M S, PARSONS G, MC NULTY M S, (1992). Characterization oj an antigenically unusual virus responsible for two outbreaks oj Newcast/ e disease in the Republic oj Ireland in 1990. Vet. Rec. 130: 65-68
ALEXANDER DJ, MANVELL RJ, LOWINGS J P, FROST K M, COLLINS M S, RUSSELL PH, SMITH J E, (1997). Antigenic diversity and similarities detected in Paramyxovirus type 1 (Newcastle disease virus) isolates using monocianal antibodies. AVian Pathol. 36: 399-418
ALLAN W H, LANCESTER J E, TOTH B, (1978). Newcastle disease vaccines: food and agriculture organizations of the United Nations. Rome.
ANDERSON C, KEARSLEY R, ALEXANDER D J, RUSSELL PH, (ı987); Antigenic variation in Avian paramyxovirus type 3 isolates detected by mouse monocianal antibodies. AVian Pathol. 16: 691-698
COLLIN M S, ALEXANDER DJ, BRACKMAN S, KEMP D A, MANVELL R S, (1989). Evaluation oj mouse monocianal antibodies raised against an isoiate oj the variant avian Paramyxovirus type 1 responsiblejor the current panzootic in pigeons. Arch. Virol. 104: 53- 61
ERDEI J, ERDEI K, KOCHIR K, KALETA E F, SHORTRIDGE K F, LOMNICCI B, (1987). Newcastle disease vaccine (Lasota) spesific monocianal antibody. Arch. Virol. 96:265-269
FEVERIO M, (1993). Newcastle disease in Portugal: Situation report- October 1993. Proceedings oj the jointjirst annual meetings oj the national Newcastle disease and Avian Injluenza Iabarataires oj the European communities. Edited by D.J.Alexander 66
HOSHI S, MIKAMI T, NAGATA K, ONUMA M, IZOWA H, (1983). Monocianal antibodies against a paramyxovirus isolatedfrom Japanase sporrow Hawlcs Arch. Virol. 85:109-121
ISHIDA M, NEROME K, MATSUMATO M, MIKAM, T, OYA A, (1985). Characterization oj rejerance strains oj Newcastle Disease virus and NDV li/ce isolates by monocianal antibodies to HN subunits. Arch . Virol. 85:112-115
IORIO RM, GLICKMAN R H, SHEEKAN P J, (1992). Inhibition oj jusion by neutralizing monocianal antibodies to HN glycoprotein oj NDV. J. Gen.Virol. 1167-1177
JESTIN V, CHERBONEL M, MORIN M, GUITTET M, BENNEJEAN G, (1989). Characterization oj French avian paramyxovirus type-1 (PMV-1) isolates with a panel oj monocianal antibodies to Plujragan strains oj NDV. Arch. Virol. 105:189-198
KALETA E F, ALEXANDER DJ, RUSSELL PH, (1985). Thejirst isolation oj the Avian PMV-1 virus responsiblejor the current panzootic in pigeons. Avian Pathol. 14 : 553-557
LANCESTER J E, ALEXANDER DJ, (1975) . Newcastle disease virus and spread, Canada Dep. Of Agriculture Monograph no11 Ottowa
MEULEMANS G, GONZE M, CARLIER M C, PETIT P, BURNY A, LONG L, (1986). Antigenic and biological characterisation oj Avian paramyxovirus type-1 isolatesfrom pigeons. Arch. Virol. 87: 151-161
MEULEMANS G, GONZE M, CARLIER M C, PETIT P, BURNY A, LONG L, (1986). Protective ejjects oj HN and F glycoprotein spesific monocianal antibodies on experimental Newcastle disease. Avian Pathol. 19:761-768
MEULEMANS G, GONZE M, CARLIER M C, PETIT P, BURNY A, LONG L, ( 1987). Evaluation oj the use oj monocianal antibodies to Hemaglutinin andjusion glycoproteins oj NDV for virus identijication and strain dijferentiation purposes. Arch. Virol. 92:55-62
NISHIKAWA K, HANADA N, MORISHIMA T, YOSHIDA T, HAMAGUCHI M, TOYODA M, NAGAI Y, (1987). Antigenic characterisation oj internal proteins oj Newcastle disease virus by monocianal antibodies. Virus research. 7 :8 3 .92
ÖNCEL T, ALEXANDER D J, MANVELL R J, TURE O, ( 1997). Characterization oj Newcastle disease virusses isolatedjrom chiclcens and pigeons in south Marmara region oj Turkey. Avian Pathal. 26:129-137
ÖZDEMİR Y, RUSSELL P H, COLLYER J, ALEXANDER D J, MANVELL RJ, (1990). Monocianal antibodies to Avian paramyxovirus type-2. Avian Pathol. 19: 395-400
RUSSELL P H, (1988). Monocianal antibodies in research, diagnosis and epizootiology oj Newcastle disease. Newcastle disease ed. by D.J.Alexander., Kluwer acad. Publ., London 137-146
RUSSELL PH, GRIFFITITHS PC, GOSWAMI KA, ALEXANDER DJ, CANNON MJ, RUSSELL W C, (1983). The characterization oj monocianal antibodies to Newcastle disease virus. J.Gen. Virol. 64:2069-2072
RUSSELL PH, ALEXANDER DJ, (1983). Antigenic variation oj Newcastle disease virus strains detected by monocianal antibodies. Ardı. Viral. 75:243-253
SRINIVASAPPA GB, SNYDER D B, MARQUART W W, KING DJ, (1986): Isolation oja monocianal antibody with a specifity for commonly employed vaccine strains oj Newcastle disease virus . Avian Dis. 562-567
UMINO Y, KOHOMA T, SATO TA, SUGIURA A, KLENK H D, ROTT R, ( 1990): Monocianal antibodies to three structural proteins of Newcastle disease virus: Biological characterization with particu/ar rejerance to the coriformational change oj envelope glycoproteins associated w ith proteolytic cleavage. J . Gen. Viral. 71:1189-1197
UMINO Y, KOHOMA T, SATO T A, SUGIURA A, (1983): Protective ejject oj monocianal antibodies to Newcastle disease virus in passive immunization. J.Gen. Viral. 71:1199-1203