Molecular detection of Eimeria stiedae in an Angora rabbit

Öz Hepatic coccidiosis caused by Eimeria stiedae, results in severe infection, outbreaks and deaths in young rabbits. Disease is diagnosed by time consuming methods like fecal examination, oocyst sporulation and necropsy. The aim of this study was to detect E.stiedae with species specific Polymerase Chain Reaction (PCR), without waiting oocyst sporulation and necropsy. Impression smears were prepared from liver nodules and stained with giemsa. Fecal samples were collected from the intestines and examined by centrifugal flotation with salt solution to detect Eimeria spp. oocysts. Then oocysts were sporulated in 2.5% (w/v) aqueous potassium dichromate (K2Cr207). Primers specific to E.stiedae were used in PCR reaction. Eimeria spp. oocysts were detected after the examination of impression smears and bowel content. E.stiedae was diagnosed after the measurement of sporulated oocyst and PCR. At the end of the study, we think that; PCR will be very valuable for the rapid and true diagnosis of hepatic coccidiosis in rabbits.

Kaynakça

1. Bhat TK, Jithendran KP, Kurade NP, (1996). Rabbit coccidiosis and its control: A review. World Rabbit Sci. 4, 37- 41.

2. Ebtesam MM, (2008). Hepatic coccidiosis of the domestic rabbit Oryctolagus cuniculus domesticus in Saudi Arabia. World J Zool. 3, 30-35.

3. Cetindag M, Biyikoglu G, (1997). The distribution of Eimeria species in domestic rabbits of Ankara region. Acta Parasitol Turcica. 21, 301-304.

4. Erdogmus ZS, Eroksuz Y, (2006). Hepatic coccidiosis in Angora Rabbits. J Anim Vet Adv. 5, 462-463.

5. Merdivenci A, (1963). Eimeria (Coccidia) species from domestic and wild rabbits in Turkey. T Biol Derg. 13, 26-35.

6. Oncel T, Gulegen E, Senlik B, Bakirci S, (2011). Intestinal coccidiosis in Angora Rabbits (Oryctolagus cuniculus) caused by Eimeria intestinalis, Eimeria perforans and Eimeria coeciola. YYU Vet Fak Derg. 22, 27-29.

7. Tasan E, Ozer E, (1989). A study on the occurrence of Eimeria (Protozoa, Eimeriidae) in hares in the vicinities of Elazig and Tunceli. Doga Turk Vet Hay Derg. 13, 60-65.

8. Cakmak A, Vatansever Z, (2001). Coccidiosis’de tanı. In: S. Dincer (Ed.): Coccidiosis. Turkiye Parazitoloji Dernegi Yayinlari, No: 17. Meta Basim, Izmir, Turkey, p.127-132.

9. Darwish AI, Golemansky V, (1991). Coccidian parasites (Coccidia: Eimeriidae) of domestic rabbits (Oryctolagus cuniculus) in Syria. Acta Protozool. 31, 209-215.

10. Hobbs RP, (1998). Coccidia (Eimeria spp) of wild rabbits in South Western Australia. Aust Vet J. 76(3), 209-210.

11. Karaer Z, (2001). Evcil Tavsanlarda (Oryctolagus cuniculus) Coccidiosis. In: S. Dincer (Ed.): Coccidiosis. Turkiye Parazitoloji Dernegi Yayinlari, No: 17. Meta Basim, Izmir, Turkey, p. 269-278.

12. Oliveira UC, Fraga JS, Licois D, Pakandl M, Gruber A, (2011). Development of molecular assays for the identification of the Eimeria species of the domestic rabbit (Oryctolagus cuniculus). Vet Parasitol. 176, 275-280.

13. Pankadl M, (2009). Coccidia of rabbit: a review. Folia Parasitol. 56, 153-166.

14. Yan W, Wang W, Wang T, Suo X, Qian W, Wang S, Fan D, (2013). Simultaneous identification of three highly pathogenic Eimeria species in rabbits using a multiplex PCR diagnostic assay based on ITS1-5.8SrRNA-ITS2 fragments. Vet Parasitol. 193, 284-288.

15. Polozowski A, (1993) Coccidiosis of rabbits and its control. Wiad Parazytol. 39, 13-28.

16. Taylor MA, Coop RL, Wall RL, (2007). Veterinary Parasitology, Third edition. Blackwell Publishing Company, Cambridge, UK, p. 605-611.

17. Long PL, Joyner LP, (1984). Problems in identification of species of Eimeria. J Protozool. 31, 535-541.

Kaynak Göster

APA Ütük, A , Pişkin, F , Balkaya, İ , Karakurum, M . (2015). Molecular detection of Eimeria stiedae in an Angora rabbit . Etlik Veteriner Mikrobiyoloji Dergisi , 26 (2) , 41-44 . DOI: 10.35864/evmd.513378