Marmara Bölgesinde Yeni Doğan Buzağı İshallerinde Bovine Coronavirusların Saptanması ve Patojenite Çalışması

Bu çalışma Marmara Bölgesi’nde yeni doğan ishalli buzağılarda BCoV’unun yaygınlığının tespiti, virus izolasyonu ve aşı virusu belirlenmesi amacıyla gerçekleştirildi. İstanbul, Tekirdağ, Kırklareli, Kocaeli illerinden sırasıyla 28, 37, 15 ve 20 adet olmak üzere toplam100 adet ishal görülen 0-2 aylık buzağıya ait gaita numunesi çalışıldı. Her bir numune örneği BCoV antijen detection ELISA ve PCR ile test edildi. Yapılan analizler sonucu gaita numunelerinden PCR testi ile 2 adet BCoV nükleik asit varlığı saptandı. Pozitif bulunan gaita örnekleri 1/10 dilüsyonlarda sulandırılıp HRT hücre kültürüne ekimleri yapıldı. Pozitif numunelerden yapılan hücre kültürü ekimlerinde BCoV izole edilemedi.

Anahtar Kelimeler:

Coronavirus, Buzağı, ELISA, izolasyon, PCR, Patojenite

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