Kuduz Laboratuvarının Günlük Çalışmaları
Bu yazıda kuduz laboratuvarının günlük çalışma mevzuu olan marazimadde muayenesi ve aşı istihsal metodları kısaca anlatılmış, ı957 -ı962 senelerinde muayene edilen marazi madde adedi, müsbet çıkanvak'alar, deneme inokülasyonu ve aşı istihsali doz olarak kaydedilmiştir.
The routine work in the Rabies Laboratory
This article is a summary of the routine works of the Rabies Laboratoryat the Etlik Veterinary Hacteriology Institute.1 - The samples that the are sent to be diagnosed from differentplaces in the country come in different forms and are subject to amethod of examination according to the form they come in.a) If the head of the animal suspected of rabies comes, or piecesof its brain in water containing 50 % glycerine, smears of thin slidescomposed of Ammon's horn are made, and stained with Seller'sdye, and Negri body are examined under the microscope. It is possiblethrough this method, to arrive at a result in a very short time.If the diagnosis could not be made with this method, the samples takenfrom different parts of the brain suspected of disease are emulsifiedin salt water and 10 % suspension is made. The suspension is inoculatedinto the 3 to 4 week - old mice. At least five rnice are used foreach sample.The mice are kept under observation for a month. The diagnosiscan be made certain by making smears from the brains of the micethat died during the above mentioned timen and examining them underthe microscope.b) It is not possible to obtain a particular result from the misroscopicexamination, if there is any putrefaction in the sample. Inthis case, the 10 % suspension made from the brain with the additionof antibiotics according to the degree of putrefaction is ~noculatedinto at least ten mice.c) If the sample has come in some kind of antiseptic, the piecestaken form the Ammon's horn are cut in microtom and stainedwith Lentz method.2 - The rabies vaccine prepared by the Sample method usingPasteur's Virus Fix is produced for the Veterinary Organization. Thebrain of either a sheep or a goat is used. The vaccine is the 10 % sus·pension of the brain made with salt water containing 0.5 % phenol.The vaccine that is prepared, after being tested for its sterility, inocutyand immunity is preserved at + 4 c degree.For preventive purposes, dogs of normal size are vaccinated twicewith a dosage of 10 ml. and small dogs and cats are vaccinated twicewith a dosage of 5 ml. with a 10 - 14 days interval between the two vaccination.In our laboratory, the vaccine prepared by avianized Kelev strainis being worked upon.The first table shows, with percentages, the number of the sampiesexamined in our laboratory during the years 1957 - 1962, and thepositive cases according to the kind of animals.The second table shows, with percentages, the number of samplessubjected to the inoculation of mice, and the positive cases.Production of vaccine has also been shown in dosage over theyears.
| APA || Güley, M . (2021). Kuduz Laboratuvarının Günlük Çalışmaları . Etlik Veteriner Mikrobiyoloji Dergisi , 2 (1) , 38-42 . Retrieved from https://dergipark.org.tr/tr/pub/evmd/issue/61156/648676 |