Ragıp BAYRAKTAR, Canan TAYHANI, M. Zafer ZOĞ, Salih ÇİÇEK

Kanatlıların IBV-Enfeksiyöz Bronşitis Hastalığına Karşı H-120 ve H-52 Suşları ile Canlı, Liyofilize, Pilot Aşı Hazırlanması

Bu araştırmada embriyolu SPF yumurtada liyofilize Mass. tipi H-120 ve H- 52 Enfeksiyöz Bronşitis virüs suşları ile aşı üretilip, kanatlıların bu hastalığa karşı kullanılması amaçlanmıştır. Canlı, liyofilize, Mass. tipi H-120 ve H-521BV suşları 9-11 günlük embriyolu SPF yumurtaya corio :ıllantoik yolla in kule edilmiş, 36-48 saatler arası amnioallantoik sıvı toplanmış , sterilite ve titre kontrolleri yapıldıktan sonra %2-3 oranında tindalize edilmiş süt tozu ilave edilmiş ve 1000 doz olacak şekilde ampullere taksim edilerek liyofilize edilen ve -20 °C'Iik deep-freeze odalarda saklanmaktadır. (20,23) Aşılar şu anda istekler doğrultusunda +4 °C'de özel ambalajlarında tüm yurda sevk edilmekte olup üretimimiz tüm ülkenin ihtiyacına cevap verecek durumdadır.

Anahtar Kelimeler:

Kanatlı, IBV-Enfeksiyöz Bronşitis Hastalığı, H-120, H-52, Ragıp BAYRAKTAR, Canan TAYHANI, M.Zafer ZOĞ, Salih ÇİÇEK, 1995, Etlik Veteriner Mikrobiyoloji Dergisi

Preparation of live Lyophilized, pilot vaccine with H-52 strain against IBV Infections Bronchitis Disease of poultry

The Study on live, Lyophilized IBV vaccines production by using IBV H- 120 and H-52 strains. In this research; Vaccine production against to lnfections Bronchitis disease had been aimed by the live, freeze-dried , Mass. type H-120 and H-52 IBV strains had been inoculated to 9-11 days old embriyoted SPF eggs by the corio-allontoic route .After 36-48 hours, the virus content. 2-3 % tyndalizated ski m med m ilk powder is ad d ed to vaccine harvest and lyophilized . Lyophilized vaccines are stored in the -20 oc deep-freeze room . (20 ,23) According to demands, the vaccines have been dispatching to all over the country, in the special boxes at +4°C.

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