Halil EROL, A. Burak GÜNGÖR, Nedret ÇELİK, Hasan KESKİN, İlkay DEMİRHAN, Hasan YURDAKUL, Elvin ÇALIŞKAN, Özden KABAKLI

İnaktif Mavidil Serotip 4 Aşısı Üretimi

Bu çalışmada, mavidil attenüe serotip 4 aşı suşu ile inaktif aşı üretimi yapıldı. Virus Vero hücre hatlarında üretilip, betapropiolactone ile inaktive edilerek, ISA 206 ( Seppic) adjuvant ile emülsifiye edildi. Aşılar daha sonra laboratuar hayvanları ve hedef hayvanlarda sterilite, toksisite ve güvenlik testleri açısından test edildi. Aşının immunojenitesi subkutan olarak 2 ml 10 koyuna, 10 keçiye ve 5 ml 3 sığıra verilerek test edildi. Rapel dozları koyunlarda 28 gün sonra yapıldı ve aşılamalar sonrası hiçbir yan etki tespit edilmedi. Aşılanan keçi, sığırların hepsinde ve koyunların %67’sinde virus spesifik antikorlar gelişti. Elde edilen bu sonuçları değerlendirmek ve minimal aşı dozunu belirlemek amacıyla bütün hedef hayvan türlerini içeren daha büyük bir hayvan grubunda yeni bir çalışma planlandı.

Anahtar Kelimeler:

Etlik Veteriner Mikrobiyoloji Dergisi, İnaktif Aşı, Mavidil, Serotip 4 Aşısı, Özden KABAKLI, A.Burak GÜNGÖR, Elvin ÇALIŞKAN, Nedret ÇELİK, İlkay DEMİRHAN, Halil EROL, Hasan KESKİN, Hasan YURDAKUL, 2011

Production of Inactivated Serotype 4 Bluetongue Vaccine

In this study production of inactivated bluetongue vaccine with attenuated bluetongue serotype-4 strain was performed. The vaccine viruses were propagated in Vero cell cultures, and inactivated with Beta-propiolactone. Subsequently by using adjuvant Montanide ISA 206 inactivation studies were carried out. Vaccines prepeared were then tested against sterility, toxicity and safety. Immunogenity of the prepeared vaccine was tested by inoculating 10 sheep and 10 goats, 3 cattle subcutonously. Booster doses were administered to sheep after 28 days. All of the vaccinated goats and cattle and 67% of the sheep showed BTV-4 specifik antibodies. To test further these promising results and to evaluate the minimum protective vaccinal dose, a new trial with a larger number of animals, including all target species, has been planned.

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