Ahmet Burak GÜNGÖR, Elvin ÇALIŞKAN, Özden KABAKLI

Attenue mavidil serotip-4 aşısının farklı stabilizatörler ile üretilmesi

Bu çalışma ile attenue mavidil serotip-4 aşısının halihazırda kullanılmakta olan embriyo ekstraktı ve yumurta sarısından oluşan stabilizatörünün yerine farklı stabilizatörlerin kullanılması ve bunların liyofilizasyon ve raf ömrüne etkileri araştırıldı. Bu amaçla Ankara Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü Viral Aşı Üretim Laboratuarı bünyesinde bulunan ve Vero hücrelerinde üretilen SA BT/4 attenüe suşu kullanıldı. Hazırlanan ham aşı sırasıyla; Lactalbumin hydrolysate %5 -sucrose %10 (LH), Weybridge Medium (%2.5 lactalbümin hyrolysate , %5 sucrose ve %1 Sodium glutamate) (WBM), Lactalbumin hydrolysate %5 -Mannitol %10 (LM), Jelatin sorbitol (hidrolize jelatin %3.5- D-sorbitol %3.5) (JS), distile suda %5 oranında Trehalose hidrate (TD) ile hazırlanan değişik stabilizatörlerle karıştırılıp liyofilizasyona tabi tutuldu. Liyofilizasyon sonucunda ortaya çıkan final ürünün kalite kontrol testleri ve raf ömrü çalışmaları gerçekleştirildi. Bu testler sonucunda; liyofilizasyon sonucunda aşı virusunda meydan gelen infektif titredeki kayıp, denenen bütün stabilizatörler için kabul edilir düzeydeydi. Denemesi yapılan stabilizatörlerle hazırlanan aşılar zararsızdı. Raf ömrü çalışmalarında en iyi performansı Lactalbumin hydrolysate %5 -sucrose %10 (LH) ile hazırlanan aşı gösterdi.

Anahtar Kelimeler:

Aşı, bağışıklık, mavidil serotip 4, raf ömrü, stabilizatör, Ahmet Burak GÜNGÖR, Özden KABAKLI, Elvin ÇALIŞKAN, 2011, Etlik Veteriner Mikrobiyoloji Dergisi

Production of attenuated live bluetongue serotype-4 vaccine with different stabilizers

In this study, effects of different stabilizers other than embryo extract and egg yolk used at present on lyophilisation and shelf life of attenuated lyophilized Bluetongue serotype-4 vaccine was investigated. For this purpose, SA BT/4 attenuated vaccine strain present at Etlik Central Veterinary Control and Research Institude Viral Vaccine Production Laboratory produced in Vero cell line is used. Bulk vaccine was mixed with 5% lactalbumin hydrolysate10% sucrose (LS), Weybridge Medium (2.5% lactalbümin hyrolysate , 5% sucrose and 1% Sodium glutamate) (WBM), 5% lactalbumin hydrolysate -10% Mannitol (LM), Gelatin sorbitole (3.5% hidrolised gelatine - 3.5% D-sorbitole) (JS) and 5% Trehalose hidrate (TD) in distilled water respectively as stabilizer. At the end of lyophilisation, quality control tests and shelf life studies of the vaccines were carried out. Results indicated that; the loss of infective titre of the vaccine virus after lyophilisation procedure was acceptable for all stabilizers, all the vaccines prepeared with mentioned stabilizers were harmless, the vaccine, prepeared with 5% lactalbumin hydrolysate-10% sucrose (LS) stabilizer had the best performance amongst in shelf life studies.

Keywords:

Bluetongue serotype 4, vaccine, stabilizer, immunity, shelf life.,

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