Dondurup Çözdürmenin Serumdaki IBR Virus Antikor Titrelerine Etkisinin SNT ve ELİSA ile Tesbiti

Bu araştırmada Türkiye'nin değişik bölgelerinden IBR/IPV şüpheli sığırlardan alınan 250 adet serum hem mikro nötralizasyon testi hemde ELISA ile test edildi. Testler arasındaki farklılık karşılaştırıldı. Ayrıca test edilen bu 250 serum içinde 44 adedi 10 kez dondurulup - çözdüri.ilerek, dondurup çözdürmenin serumlardaki antikor seviyeleri üzerine etkisi SNT ve ELISA ile araştırıldı. ELI SA ile 250 adet kan serumunun 173 adedi pozitif, 77 adedi negatif sonuç verdi. SNT ile 170 adedi pozitif, 80 adedi negatif sonuç verdi. SNT ile negatif sonuç veren 80 serumdan 3 adedi ELISA ile pozitifreaksiyon verdi . Chi square (X2) metodu kullanılarak her iki testte serumlardaki pozitiflik oranı karşılaştınldığındaX2=236(p

Anahtar Kelimeler:

IBR, Virus, Antikor, SNT, ELİSA, Antikor Titresi, Özden KABAKLI, Ayhan AKÇORA, 1996, Etlik Veteriner Mikrobiyoloji Dergisi

The Determination Of The Effect Of Freezing-Thawing On The Serum Antibody Titters For IBR Virus BY SNT and ELİSA

In this study, 250 serum samples were obtanied from cattle which infected with IBR/IPV virus, in various regions of Turkey. They were tested for IBRJ IPV virus antibodies by microneutralization test and ELISA. Two tests were compared for the detection ofthe proportion ofpositive sera. In addition to, 44 serum samples selected in 250 senım which tested before. They were frozen/ thawed 10 times and tested for effects of freezİng thawing on antibody titers in sera by SNT and ELISA. In ELISA 173 of 250 sera were estimated as positive and 77 were negative. For SNT test 170 were positive, 80 were negative. Three of 80 samples were negative for SNT and positive in the ELISA. Using the Chi square method the proportion ofpositive sera in both tests were X2=236 (p

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