Dış Alımla Sağlanan Sığırlarda İdrar Örneklerinin Clenbuterol Kalıntıları Yönünden Araştırılması

Bu çalışmada, Türkiye'ye alımı yapılan kasaplık sığırlara ait 1000 adet idrar örneği clenbuterol kalıntıları yönünden araştırıldı. Biyolojik matriks etkisini gösteren temel değerlerin saptanması ve geri kazanım çalışmalarında kullanılmak amacıyla Erzurum Et ve Balık Kurumu Kombinasında kesimi yapılan kasaplık sığırlardan elde edilen 30 adet idrar örneği kör olarak analize alındı. Çalışmada, clenbuterol'ün sığır idrarlarından pikogram düzeyinde saptanmasına olanak sağlayan hazır ticari kitler kullanılarak ELISA yöntemi uygulandı. Kör olarak deneye alınan sığır idrar örneklerinden clenbuterole eşdeğer en düşük bağlanma değeri (B/Bo) %78.2, en yüksek %88.3, ortalama %82.7 olarak saptandı. Geri kazanım çalışmaları için deneye alınan kontrol örneklerine ait sonuçlar en düşük %93 .9, en yüksek %97 .9, ortalama %95.1 olarak bulundu. Çalışmanın ikinci döneminde, Türkiye'ye alımı yapılan sığırlardan alınan 1000 adet idrar örneği clenbuterol kalıntıları yönünden araştırıldı ve sonuçlar negatif olarak bulundu.

Anahtar Kelimeler:

Etlik Mikrobiyoloji Dergisi, Clenbuterol, Kalıntı, İdrar, Sığır

The Research of Clenbuterol Residues on Samples lmported Beef Catt1e

In this study, 1000 urine samples, tak en from beef cattles w hi ch are imported by Turkey, have been examined in terms of clenbuterol residues . 30 urine samples, taken from beef cattles slaughtered in Erzurum Meat and Fish Foundation Slaughterhouse, w as taken to analyze as blank in order to be used in recovery studies and the determination of background values wich exhibit the biological matrix effect. In this study, ELISA technique was applied by use of ready commercial kits which make possible to determine clenbuterol in picogram level from cattle urine. In the blank samples, clebuterol equivalent binding values (B/B0 ) was determined as minimum 78 .2%, maximum 88.3%, mean 82.7%. The results of control samples, which was usedin recovery studies, was found to be minimum 93.9%, maximum 97 .9% and mean 95.1 %. In the second period of this study, 1000 urine samples taken from cattles which are imported by Turkey, have been examined in terms of clenbuterol residues and the results was found to be negative'.

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Anon. (1986). Corceming examination of animals and fresh meat for the precence of residues. J Eur Com L223-18.
Anon. (1988). Cumhuriyet Gazetesi İstanbul 20 Ağustos .
Baker PK, Dairymple R H, lngle D L, Ricks CA. (1989) . Use of a β adrenerjik antagonist to alter muscle and fat depositian in lams. J Anim Sci 59.
Brockway J M, Macrae J C, Williams PE V (1987). Side effects of clenbuterol asa repartitioning agent. Vet Rec 120 381-383 .
Buyse J, Deauypere E, Hjyghebaert G, Herremans M ( 1991). The effect of clenbuterol supplementation on growth performance and on plasma hormone and metabolite levels of broilers. Poultry Sci 70 993-1002.
Dairymple R H, Baker P K, Gingher D E, Ingle D L, Pensack J M, Ricks CA (1989) . A repartitioning agent to improve performance and careass composition of broilers . Poult Sci 63.
Degroodt J M, B okanski B W, Beernaert H, Courtheyn D (1989). Clenbuterol residue analysis by HPLC-HPTLC in urine and animal tissues. Lebenson unters Forsch 189 128-131.
Dumassia C M, Houghton E (1985). Secreening and Confirmatory analysis of β-agonists ~-antagonists and their metabolites in horse urine by capillary gas chromatography mass spectrometry. J. chromatography 564- 503-513.
Eddins C, Hamann J, Johnson K (1991). HPLC Analysis of clenbuterol a beta-adrenergic drug in equine urine. J . chromatographic sc i 23.
Heinrich H, Meyer D, Lucia M (1991). The Pharmacokinetics and residues of clenbuterol in veal calves . J Anim Sci 69 4538-4544.
Martinez J F (1990). Food Poisoning related to consumption of illicit β -agonist in liver. Lancet 336.
Meyer H H D, Rinko L, Dürsch I ( 1991). Residue screening for the β -agonists clenbuterol salbutamol and cimaterol in urine using enzyın immunoassay and high performan ce liquid chromatography. J chromatography 564 551-556.
Miller N P, Garcia D K, Co leman M E, Ek eren P A, Lunt D K, Wagner KA, Procknor M, Welsh T H, Smith S B (1988). Adipo~e tissue Longissimus muscle and anterior pituitary growth and fu'nction in clenbuterol fed heifers . J Anim Sci 66.
Nazzal CA (1986). The elinical pharmacology ofclenbuterol. Southem Yeterinarian 36 2 121 125.
Paleologo M O, Giacomini G, Ballaben F, Berti F, Benedetti F, Bagnati R, Bastİani E (1992). An enzyme !inked immunosorbent assay for the direct analysis of beta-agonist drugs in urine and sera. Food and Agricultural Immunology 4 73-85.
Pulce C, Lamaison D, Keck G, Bostvironnois C, Nicolas J, Descotes J (1991). Collective human food poisonings by clenbuterol residues in vealliver. Vet.Hum Toxicol 33.
Rick CA, Baker PK, Dairympte R H, Doseber M E, Ingle D L, Pankavish J (1984). Use of clenbuterol to alter mu sel e and fat accretion in swine. Fed Proc 43 . .
Wiley J (1988). A rapid liquid solid extraction procedure for the quantification of clenbuterol in urine. Biomed Environ Mass Spectrum 17 415- 416.
Yamamoto I, Iwata K, Nakashima M (1985). Pharmacokinetics of plasma and urine clenbuterol in man rat and rabbit. J Pharmacobio-Dyn 8 385-391.