Hasan Ufuk ÇELEBİOĞLU, Recep TAŞ, Hüseyin AKŞİT, Busenur ÇELEBİ

Vinca soneri'den İzole Edilen Loganic Asidin Probiyotik Bakteriler Lactobacillus acidophilus ve Lactobacillus rhamnosus'un Yüzey Hidrofobisitesi ve Oto-Agregasyonu Üzerine Etkileri

Loganic asit, çeşitli bitkilerde bulunan ve anti-enflamatuar aktivitelere sahip bir iridoid glikozittir. Bu çalışmada loganik asit, laksatif, idrar söktürücü ve antipiretik etkiler nedeniyle halk hekimliğinde kullanılan Vinca soneri bitkisinden izole edilmiştir. Bir grup fonksiyonel gıda olan probiyotikler, vücuda yeteri miktarlarda alındığında insan sağlığını olumlu yönde etkileyen mikroorganizmalardır. En çok çalışılan ve bilinen probiyotikler Lactobacillus acidophilus ve Lactobacillus rhamnosus bakterileri olmakla beraber bu bakteriler, çeşitli gıdalarda veya farklı formülasyonlarda, ayrıca besin takviyelerinde “starter” olarak kullanılır. Bu çalışmanın amacı, loganik asidin Lactobacillus acidophilus LA-5 ve Lactobacillus rhamnosus GG üzerindeki in vitro etkilerini araştırmaktır. Yüzey hidrofobisitesi ve oto-agregasyon, bir probiyotik bakteri için önemli fonksiyonlardır ve bu çalışmanın sonuçları, loganik asidin, probiyotik fonksiyonları modüle edebileceğini, dolayısıyla, loganik asit ve probiyotik suşların kombinasyonlarının daha iyi bir potansiyele sahip olabileceğini göstermiştir.

Effects of Loganic Acid isolated from Vinca soneri on Surface Hydrophobicity and AutoAggregation of Probiotic Bacteria, Lactobacillus acidophilus and Lactobacillus rhamnosus

Loganic acid is an ridoid glycoside found in various plants, having anti-inflammatory activities. In the present study, loganic acid was isolated from Vinca soneri, used in folk medicine due to laxative, diuretic and antipyretic effects. Probiotics, a group of functional foods, are microorganisms that have positive effects on human health when taken into the body in the required amounts. The most studied and known probiotics are Lactobacillus acidophilus and Lactobacillus rhamnosus. These bacteria are used as “starters” in various foods or for different formulations, as well as food supplements. The aim of this study was to investigate the in vitro effects of loganic acid on Lactobacillus acidophilus LA-5 and Lactobacillus rhamnosus GG. Surface hydrophobicity and autoaggregation are important functions for a probiotic bacterium and the results indicated that loganic acid can modulate the probiotic functions, thus there is a potential for beneficial health benefits of combinations of loganic acid and probiotic strains.

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