Fatma ŞAHİNDOKUYUCU KOCASARI, Ali BİLGİLİ

Tissue Concentrations of Sulphaquinoxaline in Healthy and E. tenella Infected Chickens

Bu çalışmanın amacı, sağlıklı ve Eimeria tenella (E. tenella) ile koksidiyoz oluşturulan etlik piliçlere tek seferde100 mg/kg.c.a dozunda verilen sülfakinoksalinin doku yoğunluklarını araştırmaktır. Çalışmada günlük, 200 adet, erkek,Avian ırkı etçi civciv kullanıldı. Hayvanlar her bir grupta 40 hayvan olacak şekilde 5 eşit gruba (Grup I, II, III, IV ve V)ayrıldı. Grup IV ve V'deki hayvanlara çalışmanın 24. gününde 10 000 E. tenella oosisti içeren inokulum verildi. İlaçbütün hayvanlara 30. günde uygulandı. İlaç Grup I'deki hayvanlara damar içi, Grup II ve Grup IV'deki hayvanlara kursak içi, Grup III ve Grup V'deki hayvanlara ise içme suyuna katılarak verildi. İlacın verilmesini takiben 1., 4., 8., 12., 18.,24., 30. ve 36. saatlerde 5'er hayvan kesildi ve hayvanların akciğer, karaciğer, böbrek, göğüs kası ve kör bağırsakdokularından örnekler alındı. Doku ilaç yoğunlukları spektrofotometrik olarak belirlendi. İlaç koksidiyozlu hayvanlarındokularında (böbrek, karaciğer, akciğer, kör bağırsak ve kas) sağlıklı hayvanlara göre daha yüksek yoğunluklardatespit edildi. İlacın en fazla biriktiği dokunun böbrek dokusu olduğu ve koksidiyozlu hayvanların kör bağırsakdokusunda sağlıklı hayvanlara göre daha yüksek yoğunlukta bulunduğu tespit edildi. Ayrıca ilacın içme suyuyla verilmesi durumunda dokulardaki yoğunluklarının kursak içine göre daha düşük olduğu belirlendi.

Sağlıklı ve Koksidiyozlu Etlik Piliçlerde Sülfakinoksalin'in Doku Yoğunlukları

The aim of this study is to investigate the tissue concentrations of sulphaquinoxaline after singleadministration of 100 mg/kg b.w. to healthy and Eimeria tenella (E. tenella) infected chickens. In this study, 200 oneday-old, male, Avian race, broiler chicks were used. The animals were divided into 5 equal groups (Group I, II, III, IVand V) and consisting of 40 chicks in each. The animals in Group IV and V were infected on 24thday with E. tenellainoculum that contains 10 000 E. tenella oocysts. The drug was applied to all chickens on the 30thday. The drug wasgiven to Group I intravenously, intracrop to Group II and Group IV and, in drinking water to Group III and to Group V.Five animals from each group were sacrificed after the drug administration at 1, 4, 8, 12, 18, 24, 30 and 36thhours andsamples were taken from the tissues of lung, liver, kidney, breast muscle and caecum of animals. The concentrationsof drug in tissue samples were measured by spectrophotometry. The level of drug was determined much higher in thetissues of chickens (kidney, liver, lung, caecum and muscle) with coccidiosis than healthy ones. The highest level ofdrug accumulation was occurred in the kidney tissue and the concentration of drug determined much higher in thecaecum tissues of coccidiosed chickens, compared to healthy counterparts. Additionally, it was found that theconcentration of the drug in tissues was lower when the drug was given in drinking water than when it was givenintracrop.

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