Sivas Yöresinde Sığır ve Kenelerde Theileria, Babesia ve Anaplasma Enfeksiyonlarının Araştırılması

Bu çalışmada, Sivas yöresinde sığır ve kenelerde Theileria, Babesia ve Anaplasma türlerinin varlığı ve yaygınlığının araştırılması amaçlandı. Sığırlardan toplanan 314 EDTAlı kan ve 610 kene örneği analiz edildi. Kan ve kenelerden elde edilen genomic DNA’lardan polimeraz zincir reaksiyonu (PZR) ile Theileria, Babesia ve Anaplasma türlerinin 18S ve 16S rRNA parsiyal gen bölgeleri amplifiye edildi. Bir membran üzerine toplam 14 prob (iki cathall/genel, iki soy ve on tür) bağlandı ve PZR ürünleri reverse line blotting (RLB) ile analiz edildi. Pozitif örneklerin 18S ve 16S rRNA genlerinin kısmi dizileri belirlendi. RLB ve sekans analizi ile Sivas yöresinde sığır ve kenelerde; T. buffeli (KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084) ve bir Babesia genotipi (Babesia sp. Sivas, KJ183081) ile bir Anaplasma genotipi (Anaplasma sp. Sivas, KJ210855) tespit edildi. Babesia sp. Sivas izolatının, B. occultans, Babesia sp. Kashi ve Babesia sp. Kayseri izolatları ile %99, Anaplasma sp. Sivas izolatının ise Anaplasma sp. Clone 7 ile %99 ve A. bovis ile %98 benzerliğe sahip olduğu belirlendi. Sığırlarda Theileria ve Anaplasma enfeksiyonlarının genel prevalansı, RLB'de sırasıyla %5.10 ve %11.15 olarak bulundu. Bu çalışma Sivas yöresinde sığırlarda ve kenelerde Theileria, Babesia ve Anaplasma türlerinin belirlendiği ilk moleküler çalışma niteliğindedir.

Anahtar Kelimeler:

Anaplasma, Babesia, kene, sığır, Sivas, Theileria

Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Region of Turkey

This study was carried out to investigate the presence and distribution of Theileria, Babesia and Anaplasma species in cattle and their ticks from Sivas region. A total of 314 EDTA-blood samples and 610 ticks were analysed. A part of 18S and 16SrRNA genes of Theileria/Babesia and Anaplasma species were amplified from the genomic DNAs extracted of the blood samples and tick pools by with polymerase chain reaction (PCR). A total of 14 probes (two catchall, two genera and ten species-specific) were bound on a membrane and then the PCR products were tested by reverse line blot (RLB) assay. The partial sequences of the 18S and 16S rRNA genes of representative positive samples were determined. According to the results of the blood and tick samples analysed by RLB and sequencing, T. buffeli (GenBank accesion number: KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084), one Babesia genotype (Babesia sp. Sivas, KJ183081) and one Anaplasma genotype (Anaplasma sp. Sivas, KJ210855) were detected. Babesia sp. Sivas were found to be 99% identical with B. occultans, Babesia sp. Kashi and Babesia sp. Kayseri whereas Anaplasma sp. Sivas were found to be 99% and 98% identical with Anaplasma sp. Clone 7 and A. bovis, respectively. Overall prevalences of Theileria and Anaplasma infections in cattle were found to be 5.10% and11.15% by RLB, respectively. This study is the first molecular survey on species of Theileria, Babesia and Anaplasma in cattle and ticks from Sivas.

Keywords:

Anaplasma, Babesia, cattle, Theileria, tick, Sivas,

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