Retikuloperitonitis travmatika ve retikuloperikarditis travmatika'lı sığırlarda bazı akut faz proteinlerin araştırılması

Bu çalısmanın amacı diagnostik öneme sahip bazı akut faz proteinlerin (AFP) retikuloperitonitis travmatika (RPT)’li ve perikarditis travmatika (PT)’li sıgırlarda belirlenmesi ve bu proteinlerin diagnostik ve prognostik önemlerinin arastırılmasıdır. Bu amaçla çalısmada 20 adet RPT’li, 6 adet PT’li ve 10 adet de saglıklı hayvan kullanıldı. RPT grubunda yer alan hayvanlara rumenotomi uygulanarak 3 gün daha hospitalize edildi. Bütün hayvanlardan 0. gün ve rumenatomi yapılanlardan da günlük olarak 3 gün daha kan örnekleri toplanarak serum amiloid A (SAA), haptoglobin (Hp), Alfa1 asit glikoprotein (1 AGP), fibrinojen (Fb) ve albumin (Alb) degerleri belirlendi. Ayrıca toplanan kan örnekleriyle gluteraldehit (GLA) testi uygulanarak her hayvan için yangı dereceleri saptandı. Yapılan analizler sonucunda RPT ve PT grubunda yer alan sıgırların siddetli derecede yangısal bir süreçte bulundugu ve bu hayvanlarda pozitif akut faz proteinlerinden Hp, SAA, 1 AGP ve Fib’in sentezinin önemli derecede arttıgı ve buna karsın negatif akut faz proteini olan Alb’in sentezinin düstügü belirlendi. Bu çalısma ile ilk defa RPT’li ve PT’li sıgırlarda akut faz proteinleri ayrıntılı olarak incelenmis ve hastalıgın patogenezi ile ilgili yeni bilgiler sunulmustur.

Investigations of some acute phase proteins in cattle with traumatic reticuloperitonitis or with traumatic pericarditis

The aim of the present study was to determine concentrations of some acute phase proteins (APP) which are significant diagnostic parameters and to investigate their prognostic and diagnostic features in cattle with traumatic reticuloperitonitis (TRP) or with traumatic pericarditis (TP). In this study, treatment groups were included 20 cattle with TRP and 6 cattle with TP, and control group was included 10 healthy cattle. Rumenotomy was performed on 13 animals of TRP group and then they were hospitalized for 3 days. Blood samples were at the base line from in 3 groups, and during the hospitilazation from 13 animals in TRP group to determine the concentrations of serum amyloid A (SAA), haptoglobin (Hp), alpha1 acid glycoprotein ( 1 AGP), fibrinogen (Fb) and albumin (Alb). Futhermore, the level of inflammation in blood was also determined by a glutaraldehyde coagulatin test (GCT). Result of the present study indicate that severe inflammation was determined, the concentrations of positive APP including Hp, SAA, 1 AGP were significantly increased, and albumin concentration was significantly decreased in cattle with TRP or TP. This is the first preliminary stuy that APP were well-investigated in cattle with TRP or TP, and also new findings were presented for their pathogenesis.

PDF

___

1. Aiello SE, 1998. Traumatic reticuloperitonitis, hemostatic disorders, haematologic reference Ranges. Aiello SE. ed. The Veterinary Merck Manual. 8th edition. Pennsylvania: Merck & Co., inc. pp. 1-2305.
2. Alsemgeest SPM, Gruy E, van der Kolk JH, Kalsbeek HC, avan Ederen AM, 1992. The plasma concentration of bovine SAA in health and disease, after surgery and endotoxin administration. Ubaldi A. ed. Vth Congrees of the ISACB Proceeding. Parma: Italy, pp.121-123.
3. Alsemgeest SPM, Kalsbeek HC, Wensing Th Koeman JP, van Ederen AM, Gruys E, 1994. Concentrations of SAA (SAA) and haptoglobin (Hp) as parameters of inflammatory diseases in cattle. Vet Quart, 16: 21-23.
4. Aslan V, Ok M, 1991. Yangı semptomu ile seyreden hastalıkların teshis ve prognozunda yeni ve basit bir test: Glutaraldehit. Türk Vet Hek Derg, 9: 24-27.
5. Aslan V, Maden M, Ok M, Basoglu A, 1993. Sıgır hastalıklarının teshis ve prognozunda kan proteinleri ve glutaraldehit testinin önemi. Doga- Türk J Vet Anim Sci, 17: 73-79.
6. Balıkçı E, Yılmaz K, 1999. Sıgırların bazı ön mide hastalıklarının tanı ve prognozunda, kan elektrolit (Na, K, Cl, Ca ve Mg) düzeyleri ve elektrokardiyogram bulgularının öneminin arastırılması. Fırat Üniv Saglık Bil Derg, 13: 349-358.
7. Balıkçı E, Günay C, 2004. Retikuloperitonitis travmatikalı sıgırlarda rumenotomi öncesi ve sonrası bazı klinik, hematolojik, biyokimyasal ve elektrokardiyografik bulguların karsılastırılması. Fırat Üniv Saglık Bil Derg, 18: 13-19.
8. Bozukluhan K, 2008. Retikuloperitonitis travmatika (RPT)’li sıgırlarda bazı akut faz proteinleri, klinik biyokimya ve hematolojik parametrelerin arastırılması. Doktora Tezi. Kafkas Üniv. Saglık Bilimleri Enstitüsü, Iç Hastalıkları Programı. Kars.
9. Braun U, Flückiger M, Nageli F, 1993. Radiography as an aid in the diagnosis of traumatic reticuloperitonitis in cattle. Vet Rec, 132: 103-109.
10. Eckersall PD, Conner JG, 1988. Bovine and Canine Acute phase proteins. Vet Res Com, 12: 169-178.
11. Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller KP, 2003. The acute phase response in calves experimentally infected with bovine viral diarrhea virus and/or Manheimia haemolytica. J Vet Med Ser, 50: 183-190.
12. Gökce HI, Gökçe G, Cihan M, 2007. Alterations in coagulation profiles and biochemical and haematological parameters in cattle with traumatic reticuloperitonitis. Vet Res Com, 31: 529-537.
13. Gruys E, Obwolo MJ, Toussaint M, 1994. Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry. A Rev Vet Bull, 64: 1009-1018.
14. Gruys E, Toussaint MJM, Niewald TA, 2005. Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci, 11: 1045-1056.
15. Heegard PMH, Godson DL, Toussaint MJM, Tjornehoj K, Larsen LE, Viuff B, Ronsholt L, 2000. The acute phase response of haptoglobin and Serum Amyloid A in cattle undergoing experimental infection with bovine respiratory syncytial virus. Vet Immunol Immunopathol, 77: 151-159.
16. Hirvonen J, Pyörala S, 1998. Acute Phase response in dairy cattle with surgically treated abdominal disorders. Vet J, 55: 53-61.
17. Horadagoda A, Eckersall PD, Hodgson JC, Gibbs HA, Moon GM, 1994. Immediate responses in serum TNF and acute phase protein concentrations to infection with P. haemolytica A1 in calves. Res Vet Sci, 57: 129-132.
18. Jafarzadeh SR, Nowrouzian I, Khaki Z, Ghamsari SM, Adibhasemi F, 2004. The sensitivities of total plasma protein and plasma fibinogen for the diagnosis of traumatic reticuloperitonitis in Cattle. Prev Vet Med, 65: 1-7.
19. Katoh N, Oikawa S, Oohashi T, Takahashi Y, Itoh F, 2002. Decreases of apolipoprotein B- 100 and A-1 concentrations and induction of haptoglobin and serum amyloid A in non-fed calves. J Vet Med Sci, 64: 51-55.
20. Kent J, 1992. Acute phase proteins; their use in veterinary diagnosis. (guest editorial) Br Vet J, 148: 279-282.