Rat Epididimal Spermasının Kısa Süreli Saklanmasında Farklı Sıcaklık Dereceleri ve Karnitinin Etkileri

Bu çalışmanın amacı, kısa süreli saklanan (0., 6., 12., 24. ve 48. saat) rat epididimal spermasında farklı saklama derecelerinin (4 oC ve 37 oC) ve karnitinin spermatolojik parametreler üzerine etkisini araştırmaktır. Araştırmada sperma donörü olarak 10 adet 8-10 haftalık yaş ve 200-250 g ağırlıklarında Wistar-Albino erişkin rat kullanıldı. Epididimisler anestezi altındaki ratlardan cerrahi teknikle alındı. Epididimal sperma numuneleri önce 4 ayrı gruba ayrıldı, numuneler Tris (T) ve Tris + 2 mM  karnitin (TC) ile sulandırıldı. Ardından sulandırılan sperma numuneleri 4 oC (T - TC) ve 37 oC (T - TC) de 48 saat süreyle kısa süreli saklandı. Saklama süresinin ardından motilite ve anormal spermatozoa oranı analiz edildi. Uygulama 10 kez tekrar edildi. Çalışmanın sonucunda, tüm deneysel gruplarda 48. saat sonunda anormal spermatozoa oranında artış ve motilite oranında düşme saptandı. Her iki sıcaklık derecesinde motilite ve spermatozoa morfolojik yapısının karnitin katılan gruplarda (TC) daha iyi korunduğu belirlendi. Sonuç olarak, 4 oC deki motilite oranı 37 oC ye kıyasla daha yüksek ve anormal spermatozoa oranı ise daha düşük bulundu. 

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Erciyes Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1304-7280
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 2004
  • Yayıncı: Erciyes Üniv. Veteriner Fak.
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