Prostaglandin E2`nin Koyunlarda Erken Luteal Dönemde Korpus Luteum Üzerine Etkisi

Prostaglandinler (PG'ler) özellikle de PGE2 luteal anjiyogenezis ve steroidogenezis’i düzenleyerek, birçok memeli türünde ovaryumun yanı sıra luteal fonksiyonun düzenlenmesinde önemli rol oynamaktadır. PGE2'nin direkt luteotropik etkisi yoğun olarak çalışılsa da PGE2'nin koyun korpus luteumundaki (CL) indirekt etkileri hakkında hala çok fazla bilgi bulunmamaktadır. Bu nedenle, bu çalışmada, korpus luteumdan izole edilen primer luteal ve mikrovasküler endotel hücreleri kullanılarak, PGE2 stimülasyonunun bazı steroidogenik hormon reseptörlerinin (ERα / ER1, ERβ / ER2 ve PGR) ve büyüme faktörlerinin (IGF1, IGF2 ve VEGF) ekspresyonu üzerindeki olası etkileri araştırılmıştır. Primer luteal hücrelerin PGE2 ile stimülasyonunu takiben ne steroidogenik hormon reseptörlerinin (ERα / ER1, ERβ / ER2 ve PGR) ne de büyüme faktörlerinin (IGF1, IGF2 ve VEGF) ekspresyonu etkilendi. Ancak, PGE2 ile primer mikrovasküler endotel hücrelerininin stimülasyonunu takiben ERα / ER1 ve ERβ / ER2`in ekspresyonu belirgin olarak arttı. Aynı zamanda, primer mikrovasküler endotel hücrelerinde IGF2’nin ekspresyonu upregüle olurken, IGF1 ve VEGF’in ekspresyonu PGE2 stimülasyonundan etkilenmedi. Sonuç olarak, PGE2 östrojen reseptörlerinin (ERα / ER1 and ERβ / ER2) ve IGF2`in ekspresyonunu düzenleyerek erken luteal dönemde luteal anjiyogenez ve vaskülogeneze katıldığı düşünülmektedir.

Anahtar Kelimeler:

Korpus luteum, koyun, prostaglandin E2

Effect of Prostaglandin E2 on Corpus Luteum Function during Early Luteal Phase in Sheep

Prostaglandins (PGs) in particular PGE2 by regulating luteal angiogenesis and steroidogenesis, play an important role in ovarian as well as luteal function in many mammalian species. While the direct luteotrophic effect of PGE2 has been studied intensively, there is still not much information available regarding the indirect effects of PGE2 in ovine corpus luteum (CL). Therefore, in the present study, possible effects of PGE2 stimulation on expression of some steroidogenic hormone receptors (ERα/ER1, ERβ/ER2 and PGR) and growth factors (IGF1, IGF2 and VEGF) were investigated using the isolated primary luteal and -microvascular endothelial cells. Neither the expression of steroidogenic hormone receptors (ERα/ER1, ERβ/ER2 and PGR) nor growth factors (IGF1, IGF2 and VEGF) were affected by PGE2 stimulation in primary luteal cells. However, there was statistically significant difference between the expression of both ERα/ER1 and ERβ/ER2 following PGE2 treatment in primary microvascular endothelial cells. Moreover, while the expression of IGF2 was upregulated, there was no statistically significant change between the expression of IGF1 and VEGF following PGE2 treatment in ovine primary microvascular endothelial cells. In conclusion, by regulating expression of estrogen receptors (ERα/ER1 and ERβ/ER2) and IGF2 in ovine luteal endothelial cells, PGE2 might involve in luteal angiogenesis and vasculogenesis during early luteal phase in sheep.

Keywords:

Corpus luteum, prostaglandin E2, sheep,

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