İbrahim AYDIN, Hacı Ahmet ÇELİK, Sait ŞENDAĞ

İneklerde prenatal embriyonik/fötal cinsiyetin belirlenmesi

Gebe ineklerde doğacak yavruların cinsiyetlerinin önceden belirlenmesi, yetiştiricilikte bazı avantajları beraberinde getirmektedir. Cinsiyet tespiti, et veya süt üretimi yapan işletmelerin üretim stratejilerini önceden planlamalarına olanak sağladığı gibi, biyoteknolojik çalışma (suni tohumlama, embriyo transferi vs.) programlarının da önceden yapılmasını kolaylaştırmaktadır. Günümüzde prenatal embriyonik ya da fötal cinsiyetin belirlenmesine yönelik çalışmalar spermatozoa, embriyo ve fötüsler üzerinde değişik yöntemler uygulanarak gerçekleştirilmektedir. Sunulan derlemede bu yöntemler hakkında ayrıntılı bilgiler verilecektir.

Prenatal embryonic or fetal determination in cows

To determine the sex of the offsprings in advance has some advantages in terms of animal breeding. To detect the sex facilitates the programming of biotechnologic studies (artificial insemination, embryo transfer etc.) as well as it helps the ones who are engaged in meat and dairy production to form their strategies in advance. Current studies regarding the determination of prenatal embryonic or fetal sex are conducted through various applications of methods on spermatozoa, embryo or fetus. In this review detailed information about these methods are given.

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1. Alaçam E, Tekeli T, Güven B, Dinç DA, Özsar S ve Güler M, 1991. İneklerde fötal sıvılardaki testosteron hormonu düzeylerinin araştırılması ile cinsiyet tayini. Hay Araş Derg., 1,1: 19-21.
2. Anderson GB, 1987. Identification of embryonic sex by detection of H-Y antigens. Theriogenology, 27: 81-97.
3. Avery B, Madison V and Greve T, 1991. Sex and development in bovine in-vitro fertilized embryos. Theriogenology, 35: 953-963.
4. Avery B, Jorgensen CB, Madison V and Greve T, 1992. Morphological development and sex of bovine in vitro-fertilized embryos. Mol Reprod Develop., 32: 265-270.
5. Binder H, Jakob CH und Bucher P, 1996. Reproduktionsmedizin im wandel: Neue entwicklungen beim embryotrarisfer. Schweizer archiv für tierheilkunde, 138, 5: 215-219.
6. Bradbury MW, Isola LM and Gordon JM, 1990. Enzymatic amplification of a Y chromosome repeat in a single blastomere allows identification of the sex of preimplantation mouse embryos. Proc Natl Acad Sci., 87: 4053-4057.
7. Bredbacka P, Bredbacka K and Peippo J, 1991. Experiences of using PCR for sexing bovine embryos. Reprod Dom Anim., 26: 75-77.
8. Carvalho RV, Del Campo MR, Palasz AT, Plante Y and Mapletoft RJ, 1996. Survival rates and sex ratio of bovine IVF embryos frozen at different developmental stages on day 7. Theriogenology, 45: 489-498.
9. Curran S, Kastelic JP and Ginther OJ, 1989. Determining sex of the bovine fetus by ultrasonic assessment of the relative location of the genital tubercle. Anim Reprod Sci., 19: 217-227.
10. Curran S and Ginther OJ, 1991. Ultrasonic determination of fetal gender in horses and cattle under farm conditions. Theriogenology, 5:809-814.
11. Dinç DA, Şendağ Ş, Çelik HA, Aydın i, Ümütlü S ve Aral F, 2002. İnek ve düvelerde real-time, B-mode ultrasonografi ile fötal cinsiyetin belirlenmesi. Vet Bil Derg., 18, (3-4): 5-11.
12. Ellis SB, Bondioli KR, Williams ME, Pryor JH and Harpold MM, 1989. Sex determination of bovine embryos using male-specific DNA probes. Theriogenology, 29: 242.
13. Espinasse J, 1990. Biotechnologien und buiatrik. Tierârztl. Umschau, 45: 666-677.
14. Franck M and Martinot S, 1993. Diagnosis of fetal sex by ultrasonography. Sciences Veterinaires Medecine Comparee, 3: 201:208.
15. Handyside AH, Penketh RJA, Winston RML, Pattinson JK, Delhanty JDA and Tuddenham EGD, 1989. Biopsy of human preiplantation embryos and sexing by DNA amplification. Lancet, 1: 347-349.
16. Herr CM, Holt NA, Matthaei Kl and Reed KC, 1990a. Sex of progeny from bovine embryos sexed with a rapid Y-chromosome-detection assay. Theriogenology, 33: 247.
17. Horvat S, Medrano JF, Behboodi E, Anderson GB and Murray JD, 1996. Sexing and detection of gene construct in microinjected bovine blastocysts using the polymerase chain reaction. Transgenic Research, 2: 134-140.
18. Jafar SI and Flint APF, 1996. Sex selection in mammals: A Review. Theriogenology, 46: 191-200.
19. Johnson LA, Cran DG and Polge C, 1994. Recent advances in sex preselection of cattle: Flow cytometric sorting of X- Y- chromosome bearing sperm based on DNA to progeny. Theriogenology, 41: 51-56.
20. Kamimura S, Nishiyama N, Ookutsu S, Goto K and Hamana K, 1997. Determination of bovine fetal sex by PCR using fetal fluid aspirated by transvaginal ultrasound-guided amniocentesis. Theriogenology, 47: 1563-1569.
21.Kahn W, 1991. Atlas und Lehrbuch der Ultraschalldiagnostik. Schlütersche, Hannover.
22. King WA, 1984. Sexing embryos by cytological methods. Theriogenology, 21:7-17.
23. King WA, 1991. Embryo-mediated pregnancy failure in cattle. Can VetJ, 32: 99-103.
24. Leibo SP and Rail WF, 1987. Determination of prenatal sex in cattle by amniocentesis. Theriogenology, 27: 246.
25. Leibo SP and Rail WF, 1990. Prenatal diagnosis of sex in bovine fetuses by amniocentesis. Theriogenology, 32: 531-551.
26. Müller E, Rath D, Klug E und Merkt H, 1986. Die anwendbarkeit der sonographie zur diagnostik am weiblichen genitale des rindes. Beri Münch Tierürztl Wschr., 99: 311 -318.
27. Müller E and Wittkowski G, 1986. Visualization of male and female characteristic of bovine fetuses by real-time ultrasonics. Theriogenology, 25: 571-574.
28. Niemann H und Meinecke B, 1993. Embryotransfer und assoziierte biotechniken bei landwirtschaftlichen nutztieren. Ferdinand Enke Verlag, Stuttgart.
29. Peippo J, Huhtinen M and Kotilainen T, 1995. ^Sex diagnosis of equine embryos using the polymerase chain reaction. Theriogenology, 44:619-627.
30. Peura T, Hyttinen JM, Trunen M and Jânne J, 1991. A reliable sex determination assay for bovine preimplantation embryos using polymerase chain reaction. Theriogenology, 35: 547-555.
31. Picard L, King WA and Betteridge KJ, 1985. Production of sexed calves from frozen-thawed embryos. Vet Rec, 117: 603-608.
32. Rail WF and Leibo SP, 1987. Production of sexed bovine pregnancies by cytogenetic analysis of cultured demi-embryos. Theriogenology, 27: 269.
33. Schwerin M, Warnat G, Giehm D, Wolf H and Thomsen PD, 1991. Multilocus genetic typing of bovine single cells by multiplex polymerase chain reaction. Reprod Dom Anim., 26: 70-74.
34. Stroud B, 1996. Using ultrasonography to determine bovine fetal sex. Food Anim Pract, 7: 663-672.
35. Thibier M and Nibart M, 1992. Bovine embryo sexing by a DNA probe on the field. Reprod Dom Anim., 27: 29-33.
36. Thibier M and Nibart M, 1995. The sexing of bovine embryos in the field. Theriogenology,43:71-80.
37. Van Vliet RA, Verrinder Gibbins AM and Walton JS, 1989. Livestock embryo sexing: A rewiev of current methods, with emphasis on Y-specific DNA probes. Theriogenology, 32:421-438.
38. Viana IG und Marx D, 1994. Geschlchtsbestimmung von rinderfeten mittels ultraschall. Tierürztl. Umschau, 49:484-486.
39. Wachtel SS, 1984. H-Y Antigen in the study of sex determination and control of sex ratio. Theriogenology, 21: 18-28.
40. White KL, Lindner GM, Anderson GB and Bondurant RH, 1982. Survival after transfer of "sexed" mouse embryos exposed to -H-Y antisera. Theriogenology, 18: 655-662.
41. White KL, Bradbury MW, Anderson GB and Bondurant RH, 1984. Immunofluorescent detection of a male-specific factor on preimplantation bovine embryos. Theriogenology, 21: 275.
42. White KL, Anderson GB and Bondurant RH, 1987. Expression of a male-specific factor on various stages of preimplantation bovine embryos. Biol Reprod., 37: 867-873.
43. Wideman D, Dorn CG and Kraemer DC, 1989. Sex detection of the bovine fetus using linear array real-time ultrasonography. Theriogenology, 31: 272.
44. Williams TJ, 1986. A technique for sexing mause embryos by a visual colorimetric assay of the X-linked enzyme, Glucose-6- Phospahate Dehydrogenase. Theriogenology, 25: 733-739.
45. Xu KP, Yadav BR, King WA and Betteridge KJ, 1992. Sex-related differences in developmental rates of bovine embryos produced and cultured in vitro. Mol Reprod Develop., 31: 249-252.