Immunologic and inflammatory reactions after safety trial in horses with DUVAXYN IE-T vaccine

Ası güvenlik testlerinde arasıra beklenmeyen bir ates ortaya çıkmaktadır. Bu çalısmanın amacı, bu ates cevabının, inaktive edilmis virus kompenetleriylemi iliskili ya da bu cevaba adjuvantın neden olup olmadıgını arastırmaktır. On adet immune edilmemis tay rastgele Grup 1 ve Grup 2 ye ayrılmıstır. Grup 1’in atları (n=5) inaktive edilmis influenza– tetanus asısı ile (Duvaxyn®IE-T) intramuskuler olarak asılanmıstır. Grup 2’nin atları ise carbomer ve aluminyumhidroksid adjuvantı içeren 'Yapmacık' (Sham) ası ile asılanmıstır. Rektal ısı test süresi boyunca ölçülmüstür. Atlardan Ilk ve ikinci asılama sonrası 0, 4 ve 12 saat ve ayrıca ikinci asılamadan 240 saat sonra kan örnekleri, atlardan alınmıstır. Protein seritleri SDS-PAGE metodu ile analize edilmistir. Test süresi boyunca, protein seritlerindeki hareketlilik ılımlı seviyede kalmıstır. Periferal beyaz kan hücrelerindeki (PWBC) sitokinlerin mRNA ekspresyonu quantitative TaqMan®Real-Time RT-PCR (qRT-PCR) teknigi ile ölçülmüstür. Sonuçta, atesin sebebi adjuvant degildir fakat ası testi boyunca görülen ates cevabı equine influenza viruslarının antijenleri ya da tetanoz toksoidi tarafından olusturulmus olabilir. PWBC deki sitokin ekspresyonu, asılamadan sonra açıkca yükselmistir. TNF- mRNA ekspresyonu ikinci asılamadan sonra birinci asılamadan sonrakine göre daha güçlü indukte edilmis görülmektedir.

Atlarda DUVAXYNIE-T aşısı ile yapılan güvenlik testinden sonraki immunolojik ve yangısal reaksiyonlar

Fever occasionally occurs with vaccine safety testing. The purpose of this study was to investigate whether this response is related to components of the inactivated virus or if it is caused by the adjuvant. Ten immune naive foals were randomly allocated to group 1 and group 2. The horses of group 1 (n=5) were intramuscularly injected with an inactivated influenza–tetanus vaccine (Duvaxyn®IE-T). The horses of group 2 (n=5) received a sham vaccine containing the carbomer and aluminiumhydroxyd adjuvant. Both groups were vaccinated second time after 4 weeks of first vaccination. Rectal temperatures were measured daily during the tests (28 days). Blood samples were taken from the horses at 0, 4, 12 hours post first and second vaccination and additionally at 240 hours after the second injection. The protein bands were analysed by SDS-PAGE methods. The fluctuation of the proteins bands was moderate over the time course. The relative mRNA expression of cytokines was measured in peripheral white blood cells (PWBC), using the quantitative TaqMan®Real-Time RT-PCR (qRT-PCR) technique. In conclusion, the adjuvant does not cause fever, but the fever response seen during vaccine testing might be caused by the antigens of equine influenza virus or the tetanus toxoid. Cytokine expression after vaccination was clearly elevated in PWBC. The mRNA-Expression of TNF- seems to be induced more strongly after the second vaccination in than after the first one.

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