Deneysel olarak oluşturulan kontrast madde nefropatisinde Arginaz, kreatinin ve üre üzerine kalpain inhibisyonunun etkisi

Bu çalışmanın amacı, kontrast madde (KM) tarafından oluşturulan kontrast madde nefropatisinde (KMN), kalpain inhibisyonunu sağlayan kalpain inhibitörü-1 (KAL-1)in karaciğer arginaz aktivitesi ile serum üre ve kreatinin düzeyleri üzerine etkilerini araştırmaktır. Çalışmada, 28 adet 8-10 haftalık Sprague Dawley cinsi dişi ratlar kullanıldı. Deney hayvanları her grupta 7 hayvan olacak şekilde 4 gruba ayrıldı: Kontrol grubu, KM verilen grup, KAL-1 verilen grup, KM ve KAL-1 verilen grup (KM+KAL-1). Kontrol grubuna herhangi bir uygulama yapılmadı. Arginaz enzim aktivitesi Tiyosemicarbazid Diasetilmonoxim Üre (TDMU) metodu, serum kreatinin düzeyi Jaffe yöntemiyle, serum üre miktarı otoanalizörde Kinetik UV Assay yöntemi ile yapıldı. Karaciğer arginaz enzim aktivitesi KM, KAL-1 ve KM+KAL-1 uygulanan ratlarda kontrol grubuna göre düşük bulundu (p

Effects of calpain inhibition on Arginase, creatinine and urea in experimental contrast medium- induced nephropathy

The purpose of this study is to investigate the activity of liver arginase and examine the effects on the levels of serum urea and creatinine of calpain inhibition (CAL-1) in contrast medium nephropathy (CMN) developed by contrast medium. Twenty-eight Sprague Dawley female rats aged 8-10 weeks were used. The experimental animals were divided into 4 groups where there were animals in each namely control, group given contrast medium (CM), group given CAL-1 (CAL-1) and group given CAL-1 and contract medium (CAL-1 + CM). No application was made to the control group. Arginase enzyme activity was measured by Tiyosemicarbazid Diacetylmonoxim Urea (TDMU) method. The level of serum creatinine was performed by Jaffe method and the amount of serum urea was performed by kinetic UV assay method in autoanalyzer. Liver arginase enzyme activity in rats given CM, CAL-1, CM + CAL-1 was found significantly low compared to the rats in the control group (p

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