Bendiokarb İnsektisitinin İnsan Lökositlerinde Lipid Peroksidasyonu, Antioksidan Enzimler ve DNA Hasarı Üzerindeki Etkileri

Bu çalışma da karbamat grubundan bir insektisit olan Bendiokarb’ın artan dozlarının insan kan hücreleri üzerine in vitro koşullarda toksik etkisini komet testi, total antioksidan kapasite tayini, antioksidan enzim aktivitesi ve Malondialdehit (MDA) seviyesini değerlendirmek suretiyle belirlemeyi amaçladık. Sigara ve alkol kullanmayan, çalıştığı ortamda herhangi bir kimyasal maddeye maruz kalmayan sağlıklı 6 erkek bireyden heparinli tüplere alınan kan örneklerinden lökosit izolasyonu yapıldı ve sonra artan dozlarda Bendiokarb’a (20, 40, 80, 160 µg/mL) maruz bırakılmıştır. Komet testi, Demir İndirgeme Antioksidan Kapasitesi (FRAP), 2-2'Azinobis 3-Ethilbenzotiazolin-6-Sülfonik Asit (ABTS/TEAC) analizleri ile antioksidan enzimler olan Süperoksit dismutaz (SOD), Katalaz (CAT), Glutatyon peroksidaz (GPx) ve lipid peroksidasyon göstergesi olan MDA düzeyleri üzerine etkileri değerlendirilmiştir. Çalışma sonunda antioksidan enzim düzeylerinin doz artışına bağlı olarak anlamlı azaldığı, tüm dozlarda ise MDA düzeylerinin anlamlı olarak arttığı belirlendi (p

Anahtar Kelimeler:

Bendiokarb, Genotoksisite, Comet Analizi, Pestisit, Antioksidan enzimler

Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes

In this study, we aimed to determine the toxic effect of increasing doses of Bendiocarb, an insecticide from carbamate group, on human blood cells in vitro via by Comet test, determination of total antioxidant capacity, antioxidant enzyme activity and malondialdehyde (MDA) level. Leukocyte isolation was performed from blood samples taken from 6 healthy male individuals who did not smoke and alcohol use and were not exposed to any chemicals in the working environment, and then exposed to increasing doses of Bendiocarb (20, 40, 80, 160 µg / mL) and Comet test, Iron Reduction Antioxidant Capacity (FRAP), 2-2'Azinobis 3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS/TEAC) analysis with Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and MDA were evaluated. At the end of the study, it was determined that antioxidant enzyme levels decreased significantly due to dose increase, whereas MDA levels increased significantly in all doses (p

Keywords:

Bendiocarb, Genotoxicity, Comet Assay, Pesticides, Antioxidant Enzymes,

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