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Zencefil (Zingiber Officinale) Kök Ekstresi Kullanılarak Çinko Oksit Nanoparçacıkların Yeşil Sentezi ve Glikoz Biyosensörü Olarak Uygulaması

Nanopartiküllerin bitki özleri yoluyla yeşil sentezi nanoteknolojide önemli bir araştırma alanı haline gelmiştir. Bu çalışmada, Zencefil kökü kullanılarak yeşil sentezlenmiş çinko oksit (ZnO) nanopartikülleri aracılığıyla yeni bir amperometrik glikoz biyosensörü üretildi. Glukoz oksidaz (GOx), glutaraldehit ile çapraz bağlanma yoluyla ZnO ile modifiye edilmiş karbon pasta elektrot (CPE) üzerine immobilize edildi. Hazırlanan biyosensör (GOx-ZnO/CPE) glikoz tayini için iyi bir elektrokatalitik özellik gösterdi. Biyosensör, düşük bir tespit limiti (14,7 μM), hızlı cevap süresi (1 saniyeden daha az), yüksek hassasiyet (15,98 µA mM−1cm−2) ve yüksek biyolojik afinite (Michaelis−Menten sabiti 0,99 mM olarak hesaplandı) gösterdi. Ayrıca hazırlanan biyosensör, askorbik asit ve ürik asit gibi girişim yapan maddelere karşı iyi bir seçicilik sergiledi. Bu sonuçlar, glikoz tayini için basit ve uygun maliyetli bir biyosensörün hazırlandığını göstermiştir.

Anahtar Kelimeler:

Yeşil Sentez, Çinko oksit nanoparçacık, Zencefil, Glikoz biyosensör

Green Synthesis of Zinc Oxide Nanoparticles Using Zingiber Officinale Root Extract and Their Applications in Glucose Biosensor

Green synthesis of nanoparticles via plant extracts has become an important research field in nanotechnology. In the present study, a novel amperometric glucose biosensor based on the green synthesized zinc oxide (ZnO) nanoparticles by using Zingiber officinale root was fabricated. Glucose oxidase (GOx) was immobilized onto the ZnO-modified carbon paste electrode (CPE) via cross-linking with glutaraldehyde. The prepared biosensor (GOx-ZnO/CPE) exhibited a good electrocatalytic ability to the determination of glucose. The biosensor also showed a low detection limit (14.7 μM), a rapid response (less than 1 second), high sensitivity (15.98 µA mM−1 cm−2), and higher biological affinity (the Michaelis−Menten constant was estimated as 0.99 mM). Moreover, the prepared biosensor exhibited good anti-interference capability in relation to ascorbic acid (AA) and uric acid (UA). These results demonstrated that a simple and a cost effectiveness biosensor was fabricated for the determination of glucose.

Keywords:

Green Synthesis, Zinc oxide nanoparticle, Zingiber Officinale, Glucose biosensor,

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