Genetic Variation in Drought Linked Morpho-physiological Characters and Microsatellite DNA Loci in Rice (Oryza sativa L.)
Genetic Variation in Drought Linked Morpho-physiological Characters and Microsatellite DNA Loci in Rice (Oryza sativa L.)
Evaluation for drought tolerance and SSR (microsatellite) markers based molecular polymorphism were investigated
in F6
plant population raised via single seed descent method from a cross between drought tolerant japonica rice
variety Azucena and drought sensitive premium traditional Basmati rice variety Taraori Basmati HBC 19. A total
of 50 F6
plants were evaluated individually for drought tolerance on 1-9 scale on the basis of agronomic characteristics,
root and shoot traits, relative water content and visual observations; the average score ranged between 1to
8.3. Fourteen plants each in the category of drought tolerant and drought sensitive were selected from F6
population
for SSR marker analysis using 30 SSR markers covering all the chromosomes. The 28 Azucena × HBC19 F6
plants
had an allele from either of the two parental lines (homozygous condition) or alleles from both the parental rice varieties
(heterozygous condition). Frequency of HBC19 specific alleles was higher in comparison to Azucena in selected
drought tolerant and drought sensitive Azucena x HBC19 F6
plants, which may be indicative of segregation distortion.
At ten SSR loci new/recombinant alleles were obtained which indicate the active recombination between genomes of
two rice varieties. Cluster tree analysis and principal component analysis demonstrate hig
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- Ahmadi N (1983) Variabilite genetique et heredite de
mecanismes de tolerance a` la secheresse chez le
riz Oryza sativa L.: I. Development du syste`me
racinaire. Agronomia Tropical 38: 110-117.
Babu RC, Nguyen BD, Chamarerek V, Shanmugasundram
P, Chezhian P, Jeyaprakash P, Ganesh
SK, Palchamy A, Sadasivam S, Sarkarung S,
Wade LJ, Nguyen HT (2003) Genetic analysis of
drought resistance in rice by molecular markers:
association between secondary traits and field
performance. Crop Science 43: 1457-1469.
Bligh HFJ (2000) Detection of adulteration of Basmati
rice with non-premium long-grain rice. International
Journal of Food Science and Technology
35: 257-265.
Bligh HFJ, Blackhall NW, Edwards KJ, McClung AM
(1999) Using Amplified Fragment length polymorphisms
and Simple Sequence Length Polymorphisms
to identify cultivars of Brown and
White Milled Rice. Crop Science 39: 1715-1721.
Brar DS, Dalmacio R, Elloran R, Aggarwal R, Angeles
R, Khush GS (1966) Gene transfer and molecular
characterization of introgression from wild
Oryza species into rice. In: Khush GS (ed) Rice
genetics III, Proceedings of 3rd International
Rice Genetics Symposium, 16-20 Oct., 1995,
IRRI, Manila, Philippines.
De Datta SK, Malabuyoc JA, Aragon EL (1988) A
field screening technique for evaluating rice
germplasm for drought tolerance during the vegetative
stage. Field Crops Research 19: 123-134.
Flowers TJ, Koyama ML, Flowers SA, Chinta Sudhakar
Singh KP, Yeo AR (2000) QTL: their place
in engineering tolerance of rice to salinity. Journal
of Experimental Botany 51(342): 99-106.
Forster BP, Ellis RP, Thomas WTB, Newton AC,
Tuberosa R, This D, ES- Enein RA, Bahri MH,
Ben-Salem M (2000) The development and application
of molecular markers for abiotic stress
tolerance in barley. Journal of Experimental
Botany 51: 19-27.
Fukai S, Cooper M (1995) Development of drought-resistant
cultivars using physio-morphological
traits in rice. Field Crops Research 40: 67-86.
Gregorio GB, Senadhira D, Mendoza RD (1997)
Screening rice for salinity tolerance. IRRI Discussion
Paper Series no. 22. Manila (Philippines):
International Rice Research Institute: 1-30.
Guiderdoni E, Corutois B, Glaszmann JC (1989) Use of
isozyme markers to monitor recombination and
assess gametic selection among anther culture derivatives
of remote crosses of rice (Oryza sativa
L.). In: Mujeeb-Kazi A. Sitch LA (ed) Review
of advances in plant biotechnology. 1985-88: 2nd
International Symposium on Genetic Manipulation
of Crops. CIMMYT and IRRI, Mexico,
D.F., Mexico and Manila, Philippines. pp 43-55.
Guvvala LD, Koradi P, Vinay S, Marella LS
(2013) Making an Indian traditional rice variety
Mahsuri, bacterial blight resistant using
marker-assisted selection. Journal of Crop Science
and Biotechnology 16: 111-121.
Hemamalini GS, Shashidhar HE, Hittalmani S (2000)
Molecular marker assisted tagging of morphological
and physiological traits under two contrasting
moisture regimes at peak vegetative stage in rice
(Oryza sativa L.). Euphytica 112: 69-78.
Ingran KT, Real JG, Maguling MA, Obien MA, Loresto
GC (1990) Comparison of selection indices
to screen lowland rice for drought resistance.
Euphytica 48: 253-260.
Jain S, Jain RK, McCouch SR (2004) Genetic analysis
of Indian aromatic and quality rice (Oryza sativa
L.) germplasm using panels of fluorescently-labeled
microsatellite markers. Theoretical and
Applied Genetics 109: 965-977.
Joseph M, Gopalakrishnan S, Sharma RK, Singh VP,
Singh AK, Singh NK, Mohapatra T (2004) Combining
bacterial blight resistance and Basmati
quality characteristics by phenotypic and molecular
marker-assisted selection in rice. Molecular
Breeding 13: 377-387.
Kanbar A, Chandrashekara M, Manjunatha K, Vinod
MS, Hittalmani S, Janamatti M, Shashidhar HE
(2004) Molecular markers for root morphological
traits under low-moisture stress using transgresant
backcrosses of rice (Oryza sativa L.).
Indian Journal of Genetics and Plant Breeding
64(3): 185-188.
Lin SY, Ikehashi H, Yanagihara S, Kawashima A
(1992) Segregation distortion via male gametes
in hybrids between indica and japonica
or wide-compatibility varieties of rice (Oryza
sativa L). Theoretical and Applied Genetics
84: 812-818.
Maekawa M, Kita F (1985) New gametophyte genes located
in the third linkage group (chromosome 3)
of rice. Japanese Journal of Breeding 35: 25-31.
3(1):25-40, 2017
40
bitki ıslahçıları alt birliği
www.bisab.org.tr
Ekin Journal
Malabuyoc JA, Aragon EL, De Datta SK (1985) Recovery
from drought-induced desiccation at the
vegetative growth stage in direct-seeded rainfed
rice. Field Crops Research 10: 105-112.
McCouch SR, Kochert G, Yu ZU, Wang ZH, Khush
GS, Coffman WR, Tanksley SD (1988) Molecular
mapping of rice chromosomes. Theoretical
and Applied Genetics 76: 815-829.
Nguyen, H.T, Babu RC, Blum A (1997) Breeding
for drought resistance in rice: physiology and
molecular genetics consideration. Crop Science
37: 1426-1434.
Olufowote JO, Xu Y, Chen X, Park WO, Beachell
HM, Dilday RH, Goto M, McCouch SR (1997)
Comparative evaluation of within-cultivar variation
of rice (Oryza sativa L.) using microsatellite
and RFLP markers. Genome 40: 370-378.
Plaschke J, Ganal M, Roder MS (1995) Detection of
genetic diversity in closely related bread wheat
using microsatellite markers. Theoretical and
Applied Genetics 91:1001–1007.
Ribaut JM, Jiang C, Gonzales-de-Leon D, Edmeades
GO, Hoisington D A (1997) Identification
of quantitative trait loci under drought conditions
in tropical maize. 2. yield components and
marker-assisted selection strategies. Theoretical
and Applied Genetics 94: 887-896.
Rohlf FJ (1990) NTSYS-PC. Numerical Taxonomy
and Multivariate Analysis System. Version 1.60.
Applied Biostatistics, New York.
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allerd
RW (1984) Ribosomal spacer length polymorphism
in barley: Mendelian inheritance,
chromosomal location and population dynamics.
Proceeding of National Academy of Sciences
USA 81: 8014-8019.
Saito A, Shimoska E, Hayano Y, Saito K, Matuura S,
Yano M (1991) Molecular mapping on chromosome
3 of rice. Japanese Journal of Breeding
41:158-159 (Suppl 1).
Sehgal D, Rajaram V, Armstead IP, Vadez V, Yadav
YP, Hash CT, Yadav RS (2012) Integration of
gene-based markers in a pearl millet genetic
map for identification of candidate genes underlying
drought tolerance quantitative trait loci.
BMC Plant Biology 12:9.
Steele KA, Price AH, Witcombe JR, Shrestha R, Singh
BN, Gibbons JM, Virk DS (2013) QTLs associated
with root traits increase yield in upland
rice when transferred through marker-assisted
selection. Theoretical and Applied Genetics
126: 101-108.
Weatherly PE (1950) Studies in the water relations
of the cotton plant I. The field measurements of
water deficts in leaves. New Physiology 49: 81.
Wu KS, Glaszmann JC, Khush GS (1988) Chromosomal
locations of ten isozyme loci in rice
(Oryza sativa L.) through trisomic analysis.
Biochemical Genetics 26:303-320.
Xiao J, Li J, Grandillo S, Ahn SN, McCouch SR,
Tanksley SD, Yuan L (1996) A wild species
contains genes that may significantly increase
the yield of rice. Nature 384: 223-224.
Yogameenakshi P, Nadarajan N, Sheeba A (2003)
Evaluation of varieties and land races for
drought tolerance in rice (Oryza sativa L.). Indian
Journal of Genetics and Plant Breeding 63
(4): 299-303.
Zhang J, Nguyen HT, Blum A (1999) Genetic analysis
of osmotic adjustment in crop plants. Journal of
Experimental Botany 50: 291-302.