Determination of Effect of Chemical Mutagen EMS on TAEK A-3 and TAEK C-10 Mutant Soybean Varieties in M1 Generation

Today beside ionizing radiation, chemical mutagens are also used in crop improvement and Ethylmethanesulphonate (EMS) is one of the most widely used chemical mutagens in the field of plant breeding. This research work was carried out to determine the effect of chemical mutagen (EMS) on seedling height. TAEK A-3 and TAEK C-10 soybean varieties were used as the test plants and effect of presoaking time determined. In greenhouse and field experiments five different EMS doses ( 0 (Control), 0,025, 0,050, 0,075 ve 1,0 M) and in greenhouse 3 different presoaking time (0, 6 and 18 hours), in the field 2 different presoaking time (0 and 6hours) were applied. After chemical mutagen application in the green house germination percentage and seedling height base EMS dose and presoaking time effect had been determined. Effects of EMS in M1 germination of soybean for different characterics (no. of plants, plant height, no of pods and seedyield) were determined under field condition. As a result it can be said that in both soybean varieties, seedling height was reduced as the EMS concentration increased. But there was no distinct influence of presoaking time on the seedling height in both soybean varieties. In both soybean varieties the average percentage of germination increased with increasing pre-soaking time. Based on results obtained in field experiment, it was concluded that by 6 hours presoaking the harmful physiolojical effect of EMS could be decreased.

Keywords:

Soybean, Induced mutation, mutation breeding, chemical mutagen EMS,

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