This study was conducted in Ankara University, Faculty of Agriculture, Department of Field crops, Biotechnology Laboratory. The objective of the present study was to determine the effects of growth regulators on tissue parameters in rice. In this study, mature embryos of three rice cultivars (Aromatik-1, Baldo and Karadeniz) and different growth regulators (2,4-Dichlorophenoxyacetic acid (2,4-D) and picloram) were used as material. For callus induction, mature embryos were placed with scutellum upwards on three different medium (hormone-free MS-0, MS + 2 mg/l 2,4-D and MS + 2.5 mg/l picloram) in sterile Petri dishes for two weeks at 25±10C in darkness. After incubation; obtained calli were transferred to hormone-free MS-0 medium for regeneration. According to results, the effect of growth regulators and genotypes on callus induction and plant regeneration in rice were found to be statistically significant