Hayat TOPCU, Nergiz COBAN, Keith WOESTE, Mehmet SUTYEMEZ, Salih Kafkas

9890

Developing new microsatellite markers in walnut (Juglans regia L.) from Juglans nigra genomic GA enriched library

Developing new microsatellite markers in walnut (Juglans regia L.) from Juglans nigra genomic GA enriched library

We attempted to develop new polymorphic SSR primer pairs in walnut using sequences derived from Juglans nigra L. genomic enriched library with GA repeat. The designed 94 SSR primer pairs were subjected to gradient PCR in 12 walnut cultivars to determine their optimum annealing temperatures and to determine whether they produce bands. Then, the primer pairs which had amplification in agarose gel were analyzed in capillary electrophoresis to determine their allele sizes. According to the gradient PCR and capillary electrophoresis results, 60.6% of the SSR primer pairs did not amplify any bands in agarose gel. Rest of the 37 primer pairs produced bands and their annealing temperatures and allele sizes were determined. From the amplified primer pairs, 18 of them were monomorphic, while 19 of them were polymorphic. As a result, 20.2% polymorphism was obtained from 94 SSR primer pairs tested in this study which had lower ratio when compared to the literature
Keywords:

SSR, walnut, polymorphism, PCR,

PDF
Ekin Journal of Crop Breeding and Genetics-Cover
  • ISSN: 2149-1275
  • Yayın Aralığı: Yılda 2 Sayı
  • Başlangıç: 2015
  • Yayıncı: Bitki Islahçıları Alt Birliği
Arşiv
Sayıdaki Diğer Makaleler

Morphological and seed yield characteristics of orchardgrass ecotypes of Eastern Anatolia Region

Pinar UYSAL, Mustafa UZUN, Mustafa Merve OZGOZ, Ayşe YAZİCİ, Kadir TERZİOGLU, Erdal AKSAKAL, Sureyya Emre DUMLU, Serafettin Cakal, Kamil HALİLOGLU

Improved vegetable varieties for Central Asia and the Caucasus developed from AVRDC-The World Vegetable Center Germplasm

Ravza F. MAVLYANOVA

Development of BAC-End based simple sequence repeat (SSR) markers in apple

Elmira Ziya MOTALEBİPOUR, Nergiz COBAN, Mortaza KHODAEİAMİNJAN, Murat GUNEY, Serif OZONGUN, Nilgun ATAY, Salih KAFKAS

Determination of morphological variability of local pea genotypes

Reyhan KARAYEL, Hatice BOZOGLU

Screening of new varieties of sainfoin with a high potential nitrogen fixation

Galina N. CHURKİNA, Evgeniya P. SALACHENOK, Galiya K. AKHMETOVA

Developing confectionery sunflower hybrids and determination of their yield performances in different environmental conditions

Veli PEKCAN, Goksel EVCİ, İbrahim M. YİLMAZ, Yalcin KAYA

Developing new microsatellite markers in walnut (Juglans regia L.) from Juglans nigra genomic GA enriched library

Hayat TOPCU, Nergiz COBAN, Keith WOESTE, Mehmet SUTYEMEZ, Salih Kafkas

The formation and study in the culture of genetic resources of forage crops by the expeditionary collection of wild forms from the natural landscapes of Kazakhstan

Meirman G. TULENDİNOVOVİCH, Yerzhanova S. TANYRBERGENOVNA

Effect of growth regulators on tissue culture parameters in rice (Oryza sativa L.)

Berk BENLİOĞLU, Duygu Ege TUNA, Melahat Avcı BİRSİN, Ahmet Murat OZGEN

A new semidwarf cultivar “Uruq” developed from irradiated stored seeds of soft wheat cv. “Inia-66”

Ayad Jaber Issa Kubba