We investigated the viability and in vitro germination of pollen in seven lemon varieties (Citrus limon (L.) Burm f.) (‘BATEM Sarısı’, ‘BATEM Pınarı’, ‘Interdonato’, ‘Kütdiken’, ‘İtalyan Memeli’, ‘Meyer’ and ‘Lamas’). The viability was tested with TTC (2, 3, 5–triphenly tetrazolium chloride) and in vitro germination was tested with agar–plate to estimate pollen viability and germination in these varieties. Additionally, effect of different sucrose concentration levels (5%, 10%, 15%, 20% and 25%) for in vitro pollen germination was observed. The results indicated that pollen of ‘Meyer’ had the highest viability with 86.74%. The pollen of ‘Lamas’ and ‘Meyer’ varieties had the highest in vitro germination with 39.77% and 39.04%, respectively. This study results showed that 20% and 25% sucrose concentrations in the agar plates had the highest germination with 36.16% and 32.12%, respectively