Diversity of Rhizobia Associated With Lablab purpureus Isolated from Algeria by PCR Amplification of the 16S rDNA PCR / RFLP
Diversity of Rhizobia Associated With Lablab purpureus Isolated from Algeria by PCR Amplification of the 16S rDNA PCR / RFLP
The objective of this study was determination of the taxonomic position of these isolates and the evaluation of the level
of approximation or divergence between these strains and the reference strains belonging to different genus of rhizobia.
Amplification of the ribosomal 16S rDNA gene (PCR / RFLP of 16S rDNA) was digested with four different restriction
enzymes: Msp I, Hinf I, Hha I and Taq I. The results of different electrophoretic profiles of fragments obtained shown the
selection of the most discriminating enzymes Msp I and Hinf I. The length polymorphism of the restriction fragments
(RFLP) analysis of PCR amplified 16S rDNA was compared with those of reference strains. Numerical analysis of
molecular characteristics showed that 20 strains studied were divided into three distinct groups; we noted that three
isolates only Lablab purpureus have a high level of similarity with the reference strain "Bradyrhizobium", while 17
isolates did not exhibit precise taxonomic status and therefore their exact phylogenetic classification is to be determined.
The nearly complete sequence of the 16S rRNA gene from a representative strain of each REP-PCR pattern showed that
the strains were closely related to the members of the family Bradyrhizobium.
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- Ando S, Yokoyama T, (1999) Phylogenetic analysis of
bradyrhizobium strains nodulating soya-bean with
reference to USDA strains of Bradyrhizobium.
Can. J. Microbiol 45:639-645.
- Bontemps C, Rogel MA, Wiechmann A, Mussabekova
A, Moody Simon, Moulin L, Elliott N, (2015)
Endemic Mimosa species from Mexico prefer
alphaproteobacterial rhizobial symbionts New.
Phytol 10:1111-13573.
- Burnie G, Gresshoff B, Bassam P, (2006) DNA
amplification fingerprinting using very short
arbitrary oligonucleotide primers Biotechnol.
9:553-559.
- Bremmer H, (1967) Soil of the Accra plains. Memoir
No 3, Soil Research Institute, Ghana Academy
of Sciences, Kumasi.
- Bringer R, Antoun H, Kloepper, J, Beauchamp J ,
(1992) Root colonization of maize and lettuce by
bioluminescent Rhizobium leguminosarum biovar
phaseoli Appl.Environ. Microbiol. 62:2767-
2772.
- Bruijn F, Voets T., Vlassak K, Vanderleyden J, Van
Rhijn P, (1992) Burkholderia tuberum sp. nov.
and Burkholderia phymatum sp. nov., nodulate
the roots of tropical legumes. Syst. Appl.
Microbial. 25:507-12.
- Day R., (1965) Particle fractionation and particlesize
analysis Methods of soil analysis, Part 1.
Agronomy 9:545-567.
- Diouf D, Fall D, Chaintreuil C, Dreyfus B, Neyra M,
Ndoye I , Moulin L, (2010) hylogenetic Panalyses
of symbiotic genes and characterization of
functional traits of Mesorhizobium spp. strains
associated with the promiscuous species Acacia
seyal Del J .Appl Microbiol 108: 818-830.
- Gyaneshwar P, Hirsch M, Moulin L, Chen W, Elliott N,
(2011) Legume-nodulating betaproteobacteria
diversity host range, and future prospects Mol
Plant Microbe. Interact. 24:1276-1288.
- Harrier M, Bedmar E, Olivares J, (1995) Host
specificity of Rhizobium strains isolated from
nitrogen-fixing trees and nitrogenase activities of
strain GRH2 symbiosis with Prosopis chilensis
Plant. Sci 42:177-182.
- Ishii S, Sadowsky M, (2009) Applications of the REPPCR
DNA fingerprinting technique to study
microbial diversity, ecology and evolution.
Environ. Microbiol. 11:733 -740.
- Jensent M, Maesen U, (1968) Cajanus DC and
Atylosia W and A (Leguminosae). In Agricultural
University Papers 854. Agricole University eds.,
Wageningen p. 225.
- Mohammed N, Willems B, Pot D, Dewettinck I,
Vandenbruaene, G, Maestrojuan B, Dreyfus K,
Kersters M, Collins M, Gillis S, (1997) Phenotypic
and genotypic characterization of bradyrhizobia
nodulating the leguminous tree Acacia Albida Int.
J. Syst. Bacteriol 44:461 473.
- Nera A, Moore E, Timmis N, (2009) Genomic
heterogeneity of strains nodulating chickpeas
(Cicer arietinum L.) and description of Rhizobium
mediterraneum sp. nov., Int. J. Syst. Bacteriol
45:640-648.
- Niemann S, Puhler A, Tichy V, Simon R, Selbitschka J,
(1997) Evaluation of the resolving power of
three different DNA fingerprinting methods to
discriminate among isolates of a natural Rhizobium
meliloti population. J. Appl. Microbiol
82:477-484.
- Norman M, Zurek T, Thanisch P, (1996) Much Ado about
Shared-Nothing SIGMOD Record. 25:16-21.
Somasegaran P, Hoben H, (1994) Handbook of Rhizobia.
Methods in Legume-Rhizobium Technology.
Springer-Verlag, New York, NY, 450 pp.
- Shetta N, Al-Shaharani T, Abdel M, (2011)
Identification and characterization of rhizobium
associated with woody legume trees grown under
Saudi Arabia condition Am. Eurasian J. Agric.
Environ.Sci 10:410-418.
- Vance C, Legocki A, Bothe H, Puhler A, (1997)
Enhanced agricultural sustainability through
biological nitrogen fixation. Biological.Nitrogen
Fixation for Ecology and Sustainable Agriculture
15:179-186.
- Walkley A, Black A, (1934) Examination of the
Degtjareff methods for determining soil organic
matter and a proposed modification of the chromic
and titration, method Soil. Sci 37: 29-38.
- Wang H, Feng T, Peng X, Yan L, Zhou L, Tang K,
(2003) Ameliorative Effects of Brassinosteroid
on Excess Manganese-Induced Oxidative Stress
in Zea mays L. Leaves. Agricultural Sciences in
China 8:1063-1074.
- Weisburg W, Barns S, Pelletier D, Lane D, (1991) 16S
ribosomal DNA amplification for phylogenetic
study J. Bacteriol. 173:697-703.
4(2):26-32, 2018
32
- Wolde-Meskel E, Terefework Z, (2005) Genetic
diversity and phylogeny of rhizobia isolated from
agroforestry legume species in southern Ethiopia.
Int. J. Syst. Evol. Microbiol 55:1439-1452.
- Yan X, Chen W, Fu J, Lu L, Xue C, (2007) Mesorhizobium
spp. are the main microsymbionts of Caagana
spp. grown in Liaoning Province of China. Microbiol
271:265-273.
- Yue Downer L, Eardly, (2011) Phylogeny of the
phototrophic Rhizobium BTAIL by polymerase
chain reaction besed sequencing of a 16S rRNA
genesegment J Bacteriol 17:2271-2277.
- Yue Li C, Jing Yu, Xin S, (2011) Bradyrhizobium
lablabi sp. nov., isolated fromeffective nodules
of Lablab purpureus and Arachis hypogaea
International Journal of Systematic and
Evolutionary Microbiology 61:2496-2502.