Behiye Banu BILGEN, Sheida DANESHVAR, Goksel EVCI, Veli PEKCAN, Mehmet İbrahim YILMAZ, Yalcin KAYA

Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers

Sunflower is one of the mos t significant oilseed crops in the world. Sunflower lines with high oleic content have highoxidative s tability of its oil. Orobanche cumana Wallr., which is a holoparasitic plant infecting the sunflower roots, isone of the limiting factors for sunflower production especially in Eas tern Europe and Turkey. Screening for high oleicacid content and broomrape resis tant sunflower genotypes by s tandard methods is time consuming and expensive.Molecular markers associated with high oleic acid trait or broomrape resis tance are useful and rapid tool in order tofacilitate sunflower breeding program. In this s tudy, two markers were chosen; SSR marker and HO PCR specificfragment for genotyping the 250 sunflower inbred lines for high oleic acid content. According to our results, high oleicacid containing hybrids expressed a specific SSR band at 246 bp, and also HO PCR specific fragment at 870 bp. Also,determination of broomrape resis tance for these 250 sunflower inbred lines was done by SCAR and SSR markers.The analyzed SCAR markers (RTS28, RTS29, RTS40 and RTS41) and SSR markers (ORS1036 and ORS1040) werelinked to the Or5 gene [provides resis tance to all five races (A-E)]. According to SCAR and SSR analysis, the s tudiedsunflower inbred lines were resis tant to races A-E. These results allowed identification of sunflower hybrids for higholeic acid traits and broomrape resis tance by DNA markers.

Keywords:

helianthus annuus, marker-assis ted selection, SCAR, SSR, oleic acid content, orobanche cumana,

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