Behiye Banu BILGEN, Sheida DANESHVAR, Goksel EVCI, Veli PEKCAN, Mehmet İbrahim YILMAZ, Yalcin KAYA

Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers

Sunflower is one of the mos t significant oilseed crops in the world. Sunflower lines with high oleic content have highoxidative s tability of its oil. Orobanche cumana Wallr., which is a holoparasitic plant infecting the sunflower roots, isone of the limiting factors for sunflower production especially in Eas tern Europe and Turkey. Screening for high oleicacid content and broomrape resis tant sunflower genotypes by s tandard methods is time consuming and expensive.Molecular markers associated with high oleic acid trait or broomrape resis tance are useful and rapid tool in order tofacilitate sunflower breeding program. In this s tudy, two markers were chosen; SSR marker and HO PCR specificfragment for genotyping the 250 sunflower inbred lines for high oleic acid content. According to our results, high oleicacid containing hybrids expressed a specific SSR band at 246 bp, and also HO PCR specific fragment at 870 bp. Also,determination of broomrape resis tance for these 250 sunflower inbred lines was done by SCAR and SSR markers.The analyzed SCAR markers (RTS28, RTS29, RTS40 and RTS41) and SSR markers (ORS1036 and ORS1040) werelinked to the Or5 gene [provides resis tance to all five races (A-E)]. According to SCAR and SSR analysis, the s tudiedsunflower inbred lines were resis tant to races A-E. These results allowed identification of sunflower hybrids for higholeic acid traits and broomrape resis tance by DNA markers.

Keywords:

helianthus annuus, marker-assis ted selection, SCAR, SSR, oleic acid content, orobanche cumana,

Tam Metin

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Bibtex	@araştırma makalesi { ekinjournal408583, journal = {Ekin Journal of Crop Breeding and Genetics}, issn = {2149-1275}, eissn = {2459-069X}, address = {Fidanlık Mah. Adakale Sok. 22/12 Kızılay/Ankara}, publisher = {Bitki Islahçıları Alt Birliği}, year = {2018}, volume = {4}, number = {1}, pages = {22 - 30}, title = {Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers}, key = {cite}, author = {Bılgen, Behiye Banu and Daneshvar, Sheida and Evcı, Goksel and Pekcan, Veli and Yılmaz, Mehmet İbrahim and Kaya, Yalcin} }
APA	Bılgen, B. B. , Daneshvar, S. , Evcı, G. , Pekcan, V. , Yılmaz, M. İ. & Kaya, Y. (2018). Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers . Ekin Journal of Crop Breeding and Genetics , 4 (1) , 22-30 .
MLA	Bılgen, B. B. , Daneshvar, S. , Evcı, G. , Pekcan, V. , Yılmaz, M. İ. , Kaya, Y. "Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers" . Ekin Journal of Crop Breeding and Genetics 4 (2018 ): 22-30 <
Chicago	Bılgen, B. B. , Daneshvar, S. , Evcı, G. , Pekcan, V. , Yılmaz, M. İ. , Kaya, Y. "Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers". Ekin Journal of Crop Breeding and Genetics 4 (2018 ): 22-30
RIS	TY - JOUR T1 - Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers AU - Behiye BanuBılgen, SheidaDaneshvar, GokselEvcı, VeliPekcan, Mehmet İbrahimYılmaz, YalcinKaya Y1 - 2018 PY - 2018 N1 - DO - T2 - Ekin Journal of Crop Breeding and Genetics JF - Journal JO - JOR SP - 22 EP - 30 VL - 4 IS - 1 SN - 2149-1275-2459-069X M3 - UR - Y2 - 2022 ER -
EndNote	%0 Ekin Journal of Crop Breeding and Genetics Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers %A Behiye Banu Bılgen , Sheida Daneshvar , Goksel Evcı , Veli Pekcan , Mehmet İbrahim Yılmaz , Yalcin Kaya %T Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers %D 2018 %J Ekin Journal of Crop Breeding and Genetics %P 2149-1275-2459-069X %V 4 %N 1 %R %U
ISNAD	Bılgen, Behiye Banu , Daneshvar, Sheida , Evcı, Goksel , Pekcan, Veli , Yılmaz, Mehmet İbrahim , Kaya, Yalcin . "Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers". Ekin Journal of Crop Breeding and Genetics 4 / 1 (Ocak 2018): 22-30 .
AMA	Bılgen B. B. , Daneshvar S. , Evcı G. , Pekcan V. , Yılmaz M. İ. , Kaya Y. Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers. Ekin Journal. 2018; 4(1): 22-30.
Vancouver	Bılgen B. B. , Daneshvar S. , Evcı G. , Pekcan V. , Yılmaz M. İ. , Kaya Y. Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers. Ekin Journal of Crop Breeding and Genetics. 2018; 4(1): 22-30.
IEEE	B. B. Bılgen , S. Daneshvar , G. Evcı , V. Pekcan , M. İ. Yılmaz ve Y. Kaya , "Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers", , c. 4, sayı. 1, ss. 22-30, Oca. 2018