A S tudy on Usability of National Regis tered Durum Wheat Cultivars for Synthetic Bread Wheat Production
A S tudy on Usability of National Regis tered Durum Wheat Cultivars for Synthetic Bread Wheat Production
The present s tudy was conducted in Agricultural Research Ins titute of the Hungarian Academy of Sciences, Martonvásár,Hungary, from 7th September 2015 to 31s t July 2016, and aimed to evaluate the crossabilities of 6 Triticum durum (Ç-1252,Kızıltan, Altıntaş, Dumlupınar Yelken and Kunduru) with 6 Aegilops tauschii (MVGB605, MVGB1323, MVGB589,CIMMYT224, CIMMYT372 and CIMMYT458). Triticum durum and Aegilops tauschii parents had been successfullygrown, and the desired number of spikes had been emasculated and pollinated (total 171 spikes). Total 34 combinationswere made and 164 embryos were obtained from 19 combinations among them. There were no embryos in 15 combinations.Number of maximum embryo rescue was obtained from Yelken/MVGB589 combination (24 embryos). It was followedby Kızıltan/MVGB589, Ç-1252/MVGB589 and Ç-1252/2T combinations (16, 13 and 11 embryos, respectively). 70germinated plants were obtained from 164 embryos in B-5 medium (in petri dishes). 94 embryos were not germinated.A total of 29 germinated embryos died in two B-5 medium (petri dish and tube). Some older and younger tillers diedafter colchicine treatment. However, we had new shooting tillers. According to results, the bes t females were Yelkenand Ç-1252 among Triticum durums, while the bes t male MVGB589, CIMMYT224 and MVGB1323 among Aegilopstauschiis, respectively.
___
- Bajaj YPS, Mahajan SK and Labana KS (1986).
Interspecific hybridization of Brassica napus
and Brassica juncea through ovary, ovule and
embryo culture. Euphytica 35: 103-109.
- Clark M and Wall W (1996). Cromosomes. Alden,
Oxford, U.K. p. 345.
- Cox TS, Raupp WJ and Gill BS (1994). Leaf rus tresis
tance genes Lr41, Lr42 and Lr43 transferred
from Triticumtauschii to common wheat. Crop
Sci. 34: 339-349.
- Damania AB(1991). Use of
genetic resources in breeding durum wheat. Plant
Breeding Abs tracts, 61: 873-881.
- Fujii S and Toriyama K (2008). Genome barriers
between nuclei and mitochondria exemplified
by cytoplasmic male s terility. Plant Cell Physiol.
49: 1484–1494.
- Gamborg OL, Miller RA and Ojima K (1968). Nutrient
requirements of suspension culture of soybean
root cells. Exp Cell Res 50:151-158.
- Kihara H (1924). Cytologische und genetische S tudien
bei wichtigen Getreidearten mit besonderer
Rücksicht auf das Verhalten der Chromosomen
und die S terilität in den Bas tarden. Mem. Cell
Sci. Kyoto Imp. Univ. Ser. B1:1-200.
- Kinoshita T (2007). Reproductive barrier and genomic
imprinting in the endosperm of flowering plants.
Genes Genet. Sys t. 82: 177-186.
- Koba T, Handa T and Shimada T (1991). Efficient
production of wheat-barley hybrids and
preferential elimination of barley chromosomes.
Theoretical and Applied Genetics, 81: 285-292.
- Linskens H (1972). The reaction of inhibition during
incompatible pollination and its elimination.
Soviet Pl. Physiol. 20, 156-166.
- McFadden ES and Sears ER (1946). The origin of
Triticum spelta and its free-threshing hexaploid
relatives. J. Hered. 37: 107-116.
- Mujeeb-Kazi A, Gul A, Farooq M, Rizwan S and
Ahmad I (2008). Rebirth of synthetic hexaploids
with global implications for wheat improvement.
Aus t. J. Agric. Res. 59: 391-398.
- Mujeep-Kazi AM, Hettel GP (1995). Utilizing Wild
Grass Biodiversity in Wheat Improvement (15
Years of Wide Cross Research at CMMYT).
Report No: 2. Mexico.
Polgari D, Cseh A, Szakacs E, Jager K, Molnar-Lang,
M and Sagi L (2014). High-frequency generation
and characterization of intergeneric hybrids and
haploids from new wheat-barley crosses. Plant
Cell Report. 33 (8), 1323-1331.
- Quısenberry KS (1967). Wheat and Wheat
Improvement. American Sosiety of Agronomy.
İnc., Publisher. Wisconsin, USA.
Shah S, Gorham J, Fors ter B and Wyn Jones RG (1987).
Salt tolerance in the Triticeae: the contribution of
the D genome to cation selectivity in hexaploid
wheat. J. Exp. Bot. 38: 254-269.
- Sharma Embryo Rescue Following Wide Crosses. In:
Hall R.D. (eds.) Plant Cell Culture Protocols.
Methods in Molecular Biology, vol 111.
Schulz A (1913). Die Geschlichte der Kultivierten
Getreide L. Neberts Verlag, Hall, Germany.
Sears ER (1956). The Transfer of Leaf Rus t Resis tance
from Aegilops umbellulata to Wheat. Brookhaven
Symp. Biol. 9:1-22.
- Sears ER (1941). Amphidiploids in the
sevenchromosome Triticinae. Mo. Agric. Expt.
S ta. Res. Bul. 336.
Sears ER (1944). The amphidiploids Aegilops
cylindrical X Triticum durum and A. Ventricosa
x T. durum and their hybrids with T.aes tivum. J.
Agric. Res. 68: 135-144.
- S tebbins GL (1958).The inviability, weakness
and s terility of interspecific hybrids. Adv. Gen.
9, 147-215.
- Thompson JP and Zwart RS (2008). Synthetic
hexaploid wheats for resis tance to root-lesion
nematodes. Proceeding of the 11th International
Wheat Genetics Symposium, 24-29 Augus t2008,
Aus tralia, 3: 849-851.
- William MDHM, Pena RJ and Mujeeb-Kazi A
(1993). Seed protein and isozyme variations in
Triticumtauschii (Aegilopssquarrosa). Theor.
Appl. Genet.87: 257-263.
- Yuan B, Cao X, and Lv A (2016). Gene introgression
from common wheat into Aegilops L, Saudi
J. Biol. Sci. doi: http://dx.doi.org/10.1016/j.
sjbs.2016.05.016.