Nihan GÜNEŞ, İsmail Can PAYLAN, Mustafa GÜMÜŞ

İzmir ilinde domates ve biber bitkilerini etkileyen tomato spotted wilt virus (TSWV) ve cucumber mosaic virus (CMV) etmenlerinin moleküler karakterizasyonu

Amaç: Bu çalışmanın amacı, İzmir'in sebze yetiştirilen önemli ilçelerinde virüs kaynaklı semptomlar gösteren domates ve biber bitkilerinde tomato spotted wilt virus (TSWV) ve cucumber mosaic virus (CMV) enfeksiyonlarının araştırılmasıdır. Materyal ve Yöntem: Domates ve biber üretim alanlarında 2019 ve 2021 yıllarında sürveyler yapılmış ve toplanan yaprak örneklerinde bu virüslerin enfeksiyon oranları RT-PCR ile belirlenmiştir. TSWV ve CMV izolatlarının GenBank veri tabanından elde edilen diğer izolatlarla benzerlik oranları ve filogenetik ilişkileri belirlenmiştir. Araştırma Bulguları: Çalışma sonucunda domates bitkilerinin aynı oranda (%21,50) TSWV ve CMV ile enfekteli olduğu belirlenmiştir. Test edilen biber örneklerinin %64,15'inin TSWV ve %25,47'sinin CMV ile enfekteli olduğu tespit edilmiştir. Sonuçlar, TSWV domates izolatlarının nükleokapsid protein gen bölgesinin nükleotid benzerlik oranının %99-96 olduğunu, TSWV biber izolatının ise %100-95 benzerlik gösterdiğini ortaya koymuştur. CMV-domates izolatının kapsid protein gen bölgesi GenBank'taki diğer izolatlarla %98-95 arasında, CMV-biber izolatınınki ise %100-98 arasında nükleotid benzerliğine sahip olmuştur. Ayrıca, CMV izolatları, alt grup IB CMV izolatları ile yakın filogenetik ilişki göstermiştir. Sonuç: Bu çalışma, İzmir ilinde simptomlu domates ve biber örneklerinde TSWV ve CMV'nin bulaşıklık oranını ve moleküler özelliklerini ortaya koymuştur.

Anahtar Kelimeler:

Biber, RT-PCR, patlıcangil, domates, virüs

Molecular characterization of tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) affecting tomato and pepper crops in Izmir Province

Objective: The objective of this study was to investigate tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) infections in tomato and pepper plants showing virus-induced symptoms in vegetable growing districts of İzmir, Turkey. Material and Methods: Surveys were carried out in tomato and pepper plantations in 2019 and 2021, and the incidences of these viruses in the collected leaf samples were determined by RT-PCR. Nucleotide identities and phylogenetic relationships of the TSWV and CMV isolates with other isolates retrieved from the GenBank database were determined. Results: The results of this study showed that tomato plants were infected at the same rate (21.50%) with TSWV and CMV. Out of the tested pepper samples, 64.15% were infected with TSWV and 25.47% with CMV. The results showed that, the identity rate of nucleoprotein region of TSWV isolates from tomato was 99-96% at nucleotide level while the isolates from pepper showed 100-95% identity. On the other hand, the capsid protein gene region of the tomato isolate of CMV had nucleotide identity rate of 98-95% with other isolates in GenBank, while that of its pepper isolates had 100-98% identity. Also, CMV isolates of this study showed close phylogenetic relationship with the CMV isolates of subgroup IB. Conclusion: This study revealed the prevalence of TSWV and CMV in symptomatic tomato and pepper samples in İzmir province and some molecular properties of them.

Keywords:

Pepper, solanaceous crops, tomato, virus, RT-PCR,

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