Nevzat SEVGİN, Aveen Khorsheed KHALİD, Merve ÖZKUL

Aktif karbon ve kültür ortamının fiziksel halinin Urmu dutu (Morus nigra L.) nun mikroçoğaltımı üzerine etkisi

Amaç: Bu çalışmanın amacı Morus nigra L. nin in vitro mikro çoğaltımı üzerinde aktif kömür ve kültür ortamının fiziksel halinin araştırılmasıdır. Materyal ve Yöntem: Çalışmada Urmu dutun (M. nigra) 3 genotipi (A5, T6, T8) explant olarak kullanılmıştır. Kültür ortamında kömürün varlığı ve ortamın fiziksel halinin (sıvı veya jel) incelenmesi için sıvı Nas ve Read medium (NRM)+0,5 gr L-1 aktif kömür, aktif kömürsüz sıvı NRM ortamı, katı NRM ortamı+0,5 gL-1 aktif kömür ve aktif kömürsüz NRM ortamı kullanılmıştır. Araştırma Bulguları: Kültür ortamında kömürün varlığı ve ortamın fiziksel hali (sıvı veya jel) bir eksplantten elde edilen ortalama sürgün sayısını önemli derecede etkilemiştir. Bir eksplantten elde edilen ortalama sürgün sayısı 1.1 ile 5.8 arasında olmuştur. Aktif kömür içermeyen ortam üzerinde daha fazla sürgün elde edilmiş ve sıvı ortama kıyasla jelleştirilmiş ortam üzerinde elde edilen sürgün sayısı daha yüksek olmuştur. Sonuç: Urmu dutunun İn vitro mikroçoğaltımında agar ile katılaştırılmış NRM ortamı sıvı NRM ortamından daha iyi sonuç vermiştir.

Anahtar Kelimeler:

Aktif kömür, in vitro katı ortam, sıvı ortam, Morus nigra L.

Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.)

Objective: The purpose of this research was to investigate the effects of charcoal and physical state of culture medium on growth of Morus nigra. L. Material and Methods: For the study tree mature black mulbery (M. nigra) genotypes A5, T6 and T8 grown in vitro were used as the explant source in this study. To find out the possible effects of charcoal and culture physical state (culture medium) on culture growth used four) Liquid Nas and Read Medium (NRM) + 0.5 gl-1 activated charcoal, Liquid NRM containing no charcoal, Gelled NRM + 0.5 gl-1 activated charcoal, agar-gelled NRM containing no charcoal) medium were used in the study to find out the possible effects of the presence of charcoal and physical state of the culture medium on culture growth. Results: Medium physical state and presence of activated charcoal in the medium greatly affected mean shoot number per cultured explants. Mean shoot numbers obtained per explants varied between 1.1 and 5.8. Medium containing no charcoal produced more shoots compared to medium containing charcoal and agar - gelled medium produced more shoots compared to liquid medium. Conclusion: Agar- The gelled medium with agar gave better results for black mulbery (M. nigra) than the liquid medium.

Keywords:

Activated charcoal in vitro, gelled medium, liquid medium, Morus nigra L.,

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