Fasulye Tohumlarındaki Viral Etmenlerin Saptanmasında Tanı Yöntemlerinin Duyarlılıklarının İncelenmesi

Anahtar Kelimeler:

Fasulye, tohum, viral enfeksiyonlar, tanılama yöntemleri

The Studies on Sensitivity of The Detection Methods of Viral Agents in Bean Seed Samples

The study was conducted to find out the presence of viral infections in 70 bean seed samples providing from different seed companies and Ege University Faculty of Agriculture Department of Horticulture. In order to determine the sensitivity of the methods used in the detection of viral infections in bean seed samples, the studies carried out in 2012 and 2013 years. To detect the viral infections in bean seed samples; symptomatology, growing-on test, biological test (mechanical inoculation), serological and molecular methods were used. The presence of 5 viral agents in bean seed samples was checked by RT-PCR, while the diagnosis of other 4 viruses was done with DAS-ELISA. Considering the results obtained, the sensitivity of the methods used in this work was investigated for the detection of viruses in bean seed samples. Out of 70 bean seed samples, the infection of certain viruses was detected in 69 samples by RT-PCR, 62 by DAS-ELISA, 48 by viral examinations and 30 by biological indexing. In growing-on assays, the viral infections were found in 48 of 64 plants developing from 70 seed samples. BCMV, BCMNV, CMV, and SMV were detected in seed samples, while AMV was not found. According to the findings from this study, it was found that RT-PCR was the most sensitive methods in the detection of the viruses in bean seed samples and the majority of bean seed samples was infected with BCMV.

Keywords:

Bean, seeds, viral infections, detection methods,

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