İzzet MUSAYEVA, Sabahattin Anıl ARI, Ali AKDEMİR, Fatih OLTULU, Çağdaş ŞAHİN, Dilek TAŞKIRAN, Aylin BUHUR, Teksin ÇIRPAN

Ratlarda oluşturulan pneumoperitoneum modelinde iskemi reperfüzyon hasarının over dokusu üzerine etkileri

Amaç: Çalışmanın amacı pneumoperitoneumun over dokusu üzerinde oluşturduğu olası iskemi reperfüzyon hasarının gösterilmesi ve D vitamininin bu hasar üzerindeki etkilerinin araştırılmasıdır. Gereç ve Yöntem: Çalışmamızda her biri 7 denekten oluşan 3 grup şeklinde Sprague Dawley cinsi dişi sıçanlar kullanılmıştır. Pneumoperitoneum sağlandıktan 60 dakika sonra desuflasyon gerçekleşt r lm ş ve 30 dakika boyunca intraabdominal basınç normalize edilmiştir eperfüzyon periyodunu takiben overler çıkarılmış ve iskemi reperfüzyon hasarı değerlend r lm şt r Histolojik olarak, overlerde foll küler dejenerasyon, vasküler konjesyon, nflamasyon, hemoraji, ödem ve DNA tamir mekanizmalarını değerlendirmek için PCNA (Proliferating cell nuclear antigen) düzeyine, biyokimyasal olarak da plazma AMH (Anti müllerian hormon) ve overde MDA (Malondialdehid), TAC (Total antioksidan kapasite) ve SOD (Süperoksid dismutaz) düzeyine bakılmıştır Bulgular: Histolojik olarak gruplar arasında farklılıklar saptanmış ve ayrıca PCNA değerleri iskemi grubunda sham ve D vitamini grubuna göre anlamlı düzeyde yüksek bulunmuştur (p

Effects of ischemia reperfusion injury on ovarian tissue in the pneumoperitoneum model created in rats

Aim: The aims of present study were revealing possible ischemia-reperfusion damage caused by pneumoperitoneum on ovarian tissue and investigating effects of vitamin D on this ovarian damage. Materials and Methods: Twenty-one adult female Sprague Dawley rats were used in this study and these rats were divided into three groups, each contained seven subjects: Sham Group, Ischemia-Reperfusion and Ischemia-Reperfusion +Vitamin D Group. In Ischemia-Reperfusion Group, desufflation was performed 60 minutes after pneumoperitoneum induction and intraabdominal pressure was normalized for 30 minutes. In the Ischemia-Reperfusion + Vitamin D group, pneumoperitoneum induction was performed after 500 mg/kg of vitamin D administration. After the reperfusion period, the ovaries were collected, and ischemia reperfusion damage was evaluated. Histochemical staining was performed to evaluate the follicular degeneration, vascular congestion, inflammation, hemorrhage and edema parameters in the ovaries. In addition, immunohistochemical PCNA (Proliferating cell nuclear antigen) staining was performed to evaluate DNA repair mechanisms. In biochemical analyzes, AMH (Anti-Mullerian hormone), MDA (Malondialdehyde), TAC (Total antioxidant capacity) and SOD (Superoxide dismutase) levels were examined in the ovaries. Results: In histological evaluation, significant differences were found between the groups and also the PCNA values were significantly higher in the Ischemia-Reperfusion Group compared to the Sham and Ischemia-Reperfusion +Vitamin D Groups (p

PDF

___

1. Akdemir A, Erbas, O, Ergenoglu M et al. Montelukast prevents ischemia/reperfusion-induced ovarian damage in rats. Eur J Obstet Gynecol Reprod Biol 2014; 173: 71–6.
2. Ergenoglu M, Erbas O, Akdemir A et al. Attenuation of ischemia/reperfusion-induced ovarian damage in rats: does edaravone offer protection. Eur Surg Res 2013; 51: 21–2.
3. Guven S, Muci E, Unsal MA et al. The effects of carbon dioxide pneumoperitoneum on ovarian blood flow, oxidative stress markers, and morphology during laparoscopy: a rabbit model. Fertil Steril 2010; 93: 1327–32.
4. Yao T, Ying X, Zhao Y, Yuan A et al. Vitamin D receptor activation protects against myocardial reperfusion injury through inhibition of apoptosis and modulation of autophagy. Antioxid Redox Signal. 2015 Mar 10; 22 (8): 633-50.
5. Xingwei X, Xin G, Peng Z et al. Low-dose ketamine pretreatment reduces oxidative damage and inflammatory response following CO2 pneumoperitoneum in rats. Clin Invest Med. 2014; 37: E124.
6. Nesek-Adam V, Mrsić V, Smiljanić A et al Pathophysiologic effects of CO2-pneumoperitoneum in laparoscopic surgery. Acta Med Croatica. 2007 Apr; 61 (2): 165-70.
7. Biler A, Yucebilgin S, Sendag F et al. The effects of different intraabdominal pressure protocols in laparoscopic procedures on oxidative stress markers and morphology in rat ovaries. Adv Clin Exp Med. 2014 Nov-Dec; 23 (6): 885-92.
8. Glantzounis GK, Yang W, Koti RS et al. The role of thiols in liver ischemia-reperfusion injury. Curr Pharm Des. 2006; 12 (23): 2891-901.
9. Kiray M, Sisman AR, Camsari UM et al. Effects of carbon dioxide exposure on early brain development in rats. Biotech Histochem. 2014 Jul; 89 (5): 371-83.
10. Yilmaz S, Koken T, Tokyol C et al. Can preconditioning reduce laparoscopy-induced tissue injury? Surg Endosc 2003; 17: 819–824.
11. Gonçalves JG, de Bragança AC, Canale D. et al. Vitamin D deficiency aggravates chronic kidney disease progression after ischemic acute kidney injury. PLoS One. 2014 Sep 15; 9 (9): e107228.
12. Uberti F, Morsanuto V, Bardelli C, Molinari C Protective effects of 1α,25-Dihydroxyvitamin D3 on cultured neural cells exposed to catalytic iron. Physiol Rep. 2016 Jun; 4 (11).