Ali ACAR, Kürşad YAPAR, Güray DEMİRTAŞ, Kültiğin ÇAVUŞOĞLU, Emine YALÇIN

Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü

Bu çalışmada albino farelerde Nigella sativa L. tohum ekstrakt (NSTE) uygulamasının Pb(NO3)2 toksisitesine karşı koruyucu rolü araştırılmıştır. Bu amaçla, fareler rastgele altı gruba ayrılmıştır. Kontrol grubu fareler çeşme suyu, uygulama grubundakiler ise 500 mg kg-1 c.a Pb(NO3)2, 300 ve 600 mg kg-1 c.a NSTE ile muamele edilmiştir. Her bir grupta total vücut ağırlığı, karaciğer ve böbrek ağırlığı belirlenerek organizma gelişimi üzerine etkiler incelenmiştir. Genotoksik etkiler, eritrosit ve yanak mukoza hücrelerinde mikronukleus (MN) sıklığı ve kemik iliği hücrelerinde kromozomal anormallikler (KA) belirlenerek saptanmıştır. Sonuç olarak Pb(NO3)2 uygulamasının vücut ağırlığında kontrol grubuna kıyasla 6.33 katlık bir azalmaya, karaciğer ve böbrek ağırlıklarında ise sırasıyla 2.08 ve 1.65 katlık bir artışa neden olduğu belirlenmiştir. NSTE uygulamasının ağırlıklarda gözlenen bu anormallikleri olumlu yönde değiştirdiği belirlenmiştir. MN analizleri sonucunda, 500 mg kg-1 Pb(NO3)2 uygulanan grupta eritrosit ve yanak mukoza hücrelerinde MN sıklığı sırasıyla 58.17±5.46 ve 19.00±2.76 olarak bulunurke, 600 mg kg-1 NSSE+Pb(NO3)2 uygulanan grupta bu değerler sırasıyla 32.67±3.78 ve 6.50±1.87 olarak bulunmuştur. NSTE’nin benzer bir koruyucu etkisi KA oluşumlarında da gözlenmiş, 600 mg kg-1 NSTE uygulamasının kromozom kırıklarını %44 oranında azalttığı belirlenmiştir. Sonuç olarak NSTE’nin kimyasalların özellikle Pb gibi ağır metallerin zararlı etkilerine karşı koruyucu bir besin olarak kullanılabileceği saptanmıştır.

In Vivo Protective Role of Nigella sativa L. Against Pb(NO3)2 Induced Toxicity

In this study, the protective role of Nigella sativa L. seed extract (NSSE) against Pb(NO3)2 toxicity in albinomice was investigated. For this purpose, the mice were randomly divided to six groups. In control group, micewere treated with tap water and in the treatment groups 500 mg kg-1bw Pb(NO3)2, 300 and 600 mg kg-1bwdoses of NSSE were exposed to mice. The effects of all treatments on organism development were investigatedby determining the changes in body, liver and kidney weights of each group. Genotoxic effects were determinedby investigating the chromosomal abnormalities (CAs) in bone marrow cells, micronucleus (MN) frequency inerythrocyte and buccal mucosa cells. As a result, it was observed that Pb(NO3)2 treatment resulted in a 6.33-folddecrease in body weight gain and 2.08 and 1.65-fold increase in liver and kidney weights compared to controlgroup, respectively. In addition, it was determined that these alterations in weights were positively changed afterNSSE treatment. From MN assays it was observed that MN frequencies of erythrocyte and buccal mucosa cellsin 500 mg kg-1bw Pb(NO3)2 treated group were found as 58.17±5.46 and 19.00±2.76 while in 600 mg kg-1bw NSSE+Pb(NO3)2 treated group, the frequencies were determined as 32.67±3.78 and 6.50±1.87,respectively. A similar protective role was also observed against CAs formation, and 600 mg kg-1 NSSEtreatment was found to reduce the chromatid breaks by 44%. As a result, it has been determined that NSSE canbe used as a protective nutrient against the harmful effects of chemicals such as heavy metals especially Pb.

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[1] N. Saglam and N. Cihangir, “The Studies on Biosorption of Heavy Metals by Biological Processes,” Hacettepe University Journal of Education, vol. 11, pp. 157-161, 1995.
[2] P. B. Tchounwou, C. G. Yedjou, A. K. Patlolla and D. J. Sutton, “Heavy Metal Toxicity and the Environment,” Molecular, Clinical and Environmental Toxicology, vol. 101, pp. 133-164, 2012.
[3] J. A. Centeno, R. B. Finkelman and O. Selinus, “Medical Geology: Impacts of the Natural Environment on Public Health,” Geosciences, vol. 4, pp. 114-127, 2014.
[4] H. Gurer and N. Ercal, “Can Antioxidants be Beneficial in the Treatment of Lead Poisoning?,” Free Radical Biology and Medicine, vol. 29, no. 10, pp. 927-945, 2000.
[5] B. Seven, E. Yalcin, A. Acar, K. Yapar and K. Cavusoglu, “Investigation of Genotoxicity Caused by Paraben in Albino Mice: Protective Role of Nettle Extract,” Cumhuriyet Science Journal, vol. 38, no. 3, pp. 572-580, 2017.
[6] E. Yalcin, A. Acar and K. Cavusoglu, “Investigation of the Protective Role of Carotene Against Toxicity Encouraged by Ammonium Sulfate in Albino Mice,” Düzce University Journal of Science & Technology, vol. 5, no. 1, pp. 273-280, 2017.
[7] M. Burits and F. Bucar, “Antioxidant Activity of Nigella sativa Essential Oil,” Phytotherapy Research, vol. 14, no. 5, pp. 323-328, 2000.
[8] C. Nergiz and S. Otles, “Chemical Composition of Nigella sativa L. Seeds,” Food Chemistry, vol. 48, no. 3, pp. 259-261, 1993.
[9] M. Mahfouz and M. El-Dakhakhny, “The Isolation of a Crystalline Active Principle from Nigella sativa L. Seeds,” Journal of Pharmaceutical Sciences, vol. 1, pp. 1-19, 1960.
[10] International Ethical Guidelines for Epidemiological Studies” repared by the Council for International Organizations of Medical Sciences (CIOMS) in collaboration with the World Health Organization (WHO). February 2008. Geneva.
[11] T. H. Ma, X. Zhou, G. F. Loarco, G. G. Arreola and S. U. Lecona, “Mouse–Erythrocyte Micronucleus (Mus-EMN) Assay on the Clastogenicity of Industrial Wastewater,” Rev. Int. Contam. Ambient, vol. 11, no. 2, pp. 95-98, 1995.
[12] M. Fenech, W. P. Chang, M. Kirsch-Volders, N. Holland, S. Bonassi and E. Zeiger, “HUMN project: Detailed Description of the Scoring Criteria for the Cytokinesis-block Micronucleus Assay Using Isolated Human Lymphocyte Cultures,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 534, no. 1-2, pp. 65-75, 2003.
[13] J. R. Savage, “Classification and Relationships of Induced Chromosomal Structual Changes,” Journal of Medical Genetics, vol. 13, no. 2, pp. 103-122, 1976.
[14] S. J. S. Flora, M. Mittal and A. Mehta, “Heavy Metal Induced Oxidative Stress & Its Possible Reversal by Chelation Therapy,” Indian Journal of Medical Research, vol. 128, no. 4, pp. 501-523, 2008.
[15] K. P. Woodward. “Growth, Cellular Development of Skeletal Muscle and Body Composition of Rats Selected for Post Weaning gain,” Retrospective Theses and Dissertations, vol. 6254, 1976.
[16] T. C. King. “Cell Injury, Cellular Responses to Injury and Cell Death,” in Elsevier's Integrated Pathology, 2007.
[17] A. P. Yagminas, C. A. Franklin, D. C. Villeneuve, A. P. Gilman, P. B. Little and V. E. O. Valli, “Subchronic Oral Toxicity of Triethyl Lead in the Male Weanling Rat. Clinical, Biochemical, Hematological, and Histopathological Effects,” Toxicological Sciences, vol. 15, no. 3, pp. 580-596, 1990.
[18] A. Ahmad, A. Husain, M. Mujeeb, S. A. Khan, A. K. Najmi, N. A. Siddique, Z. A. Damanhouri and F. Anwar, “A Review on Therapeutic Potential of Nigella sativa: A Miracle Herb,” Asian Pacific Journal of Tropical Biomedicine, vol. 3, no. 5, pp. 337-352, 2013.
[19] J. T. Tapisso, C. C. Marques, M. da Luz Mathias and M. da Graca Ramalhinho, “Induction of Micronuclei and Sister Chromatid Exchange in Bone-Marrow Cells and Abnormalities in Sperm of Algerian Mice (Mus spretus) Exposed to Cadmium, Lead and Zinc,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 678, no. 1, pp. 59-64, 2009.
[20] G. C. Jagetia and R. Aruna, “Effect of Various Concentrations of Lead Nitrate on the Induction of Micronuclei in Mouse Bone Marrow,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 415, no. 1, 131-137, 1998.
[21] M. Von Ledebur and W. Schmid, “The Micronucleus Test Methodological Aspects,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 19, no. 1, pp. 109-117, 1973.
[22] K. Mortelmans, and D. S. Rupa, “Current Issues in Genetic Toxicology Testing for Microbiologists,” Advances in Applied Microbiology, vol. 56, no. 3, pp. 379-397, 2004.
[23] E. I. Aboul-Ela, “Cytogenetic Studies on Nigella sativa Seeds Extract and Thymoquinone on Mouse Cells Infected with Schistosomiasis Using Karyotyping,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 516, no. 1, pp. 11-17, 2002.
[24] A. Kasperczyk, L. Słowińska-Łożyńska, M. Dobrakowski, J. Zalejska-Fiolka and S. Kasperczyk, “The Effect of Lead-Induced Oxidative Stress on Blood Viscosity and Rheological Properties of Erythrocytes in Lead Exposed Humans,” Clinical Hemorheology and Microcirculation, vol. 56, no. 3, pp. 187-195, 2014.
[25] S. Nehar and M. Kumari. “Ameliorating Effect of Nigella sativa oil on Thioacetamideinduced Liver Cirrohsis in Albino Rats,” Indian Journal of Pharmaceutical Education and Research, vol. 47, pp. 135-139, 2013.
[26] D. Canayakin, Y. Bayir, N. Kilic Baygutalp, K. Sezen, E. Karaoglan, H. Atmaca, F.B. Kocak Ozgeris, M.S. Keles and Z. Halici. “Paracetamol-induced nephrotoxicity and oxidative stress in rats: the protective role of Nigella sativa,” Pharmaceutical Biology, vol. 54, no. 10, pp. 2082-2091, 2016.
[27] A. Mabrouk and H. Cheikh. “Thymoquinone supplementation reverses lead-induced oxidative stress in adult rat testes,” General Physiology and Biophysics, vol. 34, pp.65–72, 2015.
[28] M. J. Salomi, S. C. Nair and K. R. Panikkar, “Inhibitory Effects of Nigella sativa and Saffron (Crocus sativus) on Chemical Carcinogenesis in Mice,” Nutrition and Cancer, vol. 16, no. 1, pp. 67- 72, 1991.
[29] M. Tariq, “Nigella sativa Seeds: Folklore Treatment in Modern Day Medicine,” Saudi Journal of Gastroenterology, vol. 14, no. 3, pp. 105-106, 2008.
[30] E. Lev and Z. Amar, “Ethnopharmacological Survey of Traditional Drugs Sold in Israel at the end of the 20th Century,” Journal of Ethnopharmacology, vol. 72, no. 1-2, pp. 191-205, 2000.
[31] O. A. Badary, R. A. Taha, A. M. Gamal El-Din and M. H. Abdel-Wahab, “Thymoquinone is a Potent Superoxide Anion Scavenger,” Drug and Chemical Toxicology, vol. 26, no. 2, pp. 87-98, 2003.