Elektrokimyasal Biyosensörlerin Geliştirilmesinde Alkanetiol Kendiliğinden Oluşan Tek Katmanlar ile Peptid Nükleik Asit (PNA) Probu

Peptid Nükleik Asit (PNA) içeren alkanetiol kendiliğinden oluşan tek katmanlar (SAM), elektrokimyasal biyosensörlerde prob olarak önerilmiştir. Bu makale, PNA probunun optimizasyonunu iki farklı SAM konsantrasyonu, immobilizasyon sıcaklığı ve iki aşamalı immobilizasyon prosesi kullanarak araştırmıştır. Alkanetiol kendiliğinden oluşan tek katmanlar ile PNA prob immobilizasyonu ve cDNA (tamamlayıcı DNA) hibridizasyonu AC voltametre metodu ile ölçülmüştür. Yüksek iyonik kuvvet konsantrasyonunda, cDNA hibridizasyonundan sonra formal potansiyelde küçük negatif kayma (-17 mV) meydana gelmiştir

Anahtar Kelimeler:

Alkanetiol kendiliğinden oluşan tek katmanlar, PNA, Elektrokimyasal biyosensörler, AC voltametre

Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors

Alkanethiol self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed as a novel probe design in electrochemical biosensors. This paper investigates the optimization of PNA probe by using two different concentrations of SAMs, immobilization temperature, and two-stage immobilization process. Alkanethiol self-assembled monolayers with PNA probe immobilization and cDNA (complementary DNA) hybridization were measured via AC voltammetry method. The small negative shift in formal potential (-17 mV) was occurred after cDNA hybridization at high ionic strength concentration.

Keywords:

Alkanethiol self-assembled monolayers, PNA, electrochemical biosensors, AC voltammetry,

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