Nesibe YAMAK, Murat OKTAY, Emine YAYKAŞLI, Elif ÖNDER, Nilay Aydin OKTAY, Yusuf AYDIN, Düzce Atatürk DEVLET, Kürşat Oğuz YAYKAŞLI

Papiller Tiroid Kanserinde Braf T1799a Mutasyonunun Tümör Büyüklüğüne Etkisi

Amaç: Tiroid kanseri en yaygın endokrin malinitesidir ve tüm dünyada insidansı giderekartmaktadır. Tüm tiroid kanseri vakalarının yaklaşık % 80’ini teşkil eden papiller tiroidkanserinin etyolojisinde başta T1799A mutasyonu olmak üzere birçok BRAF genmutasyonlarının yer aldığı bilinmektedir. Bu çalışmanın amacı BRAF T1799A mutasyonuntiroid kanserindeki frekansı ve tümör büyüklüğü ile muhtemel ilişkisini araştırmaktır.Gereç ve Yöntem: Çalışmaya tiroid kanseri tanısı konmuş 58 olgu ve kanserden başkasebeplerle opere edilmiş 52 olgu olmak üzere toplam 108 olgu dahil edilmiştir. Olgularınparafine gömülü dokularından DNA izole edilerek genotipleme PCR-RFLP yöntemiyle analizedilmiştir. İstatistiksel analizler SPSS 17.0 programı kullanılarak yapılmıştır. 0,05’ten küçükp değerleri anlamlı olarak kabul edilmiştir.Bulgular: Tiroid kanseri ile kontrol grupları arasında AA genotipi açısından istatistiksel biranlam bulunamazken TA genotipi açısından istatistiksel olarak anlamlı bir ilişki tespit edildi(p=0,0001, OR=7,089 (2,7-18,6)). Tümör boyutu 1 cm’den küçük ve büyük olan papiller tiroidkanseri grupları arasında hem TA genotipi (p=0,51, OR=0,641 (0,1-2,4)) hem de AA genotipi(p=0,67, OR=0,694 (0,1-3,8)) açısından istatistiksel olarak anlamlı bir ilişki bulunamadı.Sonuç: Tiroid kanseri diğer kanser türleri arasında en fazla artış hızına sahip olduğundanmoleküler temelinin aydınlatılması önem kazanmıştır. Yapılan bu çalışmada en yaygın görülenBRAF T1799A mutasyonun tümör büyüklüğüne etkisinin olmadığı sonucuna varılmıştır. Fakatdaha net sonuçlar alabilmek için çalışma daha büyük popülasyonlarda tekrarlanmalıdır

Anahtar Kelimeler:

Papiller tiroid kanseri, BRAF, tümör boyutu

The Effect of BRAF T1799A Mutation on Tumor Size of Papillary Thyroid Cancer

Objective: The incidence of thyroid cancer, the most common endocrine malignancy isincreasing in all populations. Papillary thyroid cancer constitutes approximatelly 80 % of thethyroid cancers and several BRAF gene mutations escpecially T1799A are involved in theetiology of papillary thyroid cancer. The aim of this study was to investigate the frequency ofBRAF T1799A mutation in thyroid cancer and the possible association between BRAFT1799A mutation and tumor size.Methods: The study was conducted with 58 cases with thyroid cancer and 52 cases with nocancer in totally 108 cases. DNA isolated from paraffin-embedded tissues of cases, and thegenotypes were analyzed by PCR-RFLP method. Statistical analyses were performed usingSPSS 17.0 software. P values less than 0.05 were accepted as statistically significant.Results: There was no statistically significant association between patient and control groupsin term of the AA genotype. However TA genotype was significantly higher in patient groupthan control group (p=0.0001, OR=7.089 (2.7-18.6)). When comparing less and more than 1cm tumor size groups in papillary thyroid cancer cases, there was no statistically significantassociation for TA genotype (p=0,51, OR=0,641 (0,1-2,4)) and AA genotype (p=0,67,OR=0,694 (0,1-3,8)).Conclusion: Thyroid cancer has the most increasing rate among all cancers types in the world.So, the elucidation of the molecular basis in detail has gained the great importance. In thisstudy, it was concluded that BRAF T1799A mutation has no effect on tumor size. However,this study should be repeated with larger population to get more confident results

Keywords:

Papillary throid cancer, BRAF, tumor size,

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