Nodüler Guatr Tanısı Almış Hastaların Tiroid Hücrelerinin AgNOR Sayısı / Çekirdek ve Toplam AgNOR Alanı / Çekirdek Alanı Oranının Değerlendirilmesi

Amaç: Çekirdekçik organize edici bölgeler (NORs) çekirdekçik içerisinde preribozomlarınişlenerek sitoplazmada olgun ribozom parçalarını oluşturan, ribozomal RNA (rRNA)’laratranskribe edilen ve tandem olarak tekrarlanan DNA ilmekleridir. Bu merkezler gümüş boyamasonrasında incelendiğinde, interfaz çekirdeğinde görülebilirler. Bu çalışmanın amacı, nodülerguatır tanısı almış bireylerin tiroid hücrelerindeki ortalama AgNOR sayısı (herbir kişide toplamAgNOR sayısı / incelenen çekirdek sayısı oranı) ve “Toplam AgNOR alanı / çekirdek alanı”(TNA / ÇA) oranlarının cinsiyet ve yaşa göre değişip değişmediğinin araştırılmasıdır.Gereç Yöntem: Çalışmaya ince iğne aspirasyon biyopsisi (İİAB) nodüler guatr ile uyumlu olan(kistik olmayan) 15 Kadın (yaş aralığı 38–68) ve 15 erkek (yaş aralığı 38–68) birey dahil edildi.İİAB ile elde edilen biyopsi materyalleri, AgNOR’ların tespiti için spesifik bir protokole göreboyandı. Her bir birey için 100 çekirdek değerlendirilerek, ortalama AgNOR sayısı ve ‘‘TNA /ÇA’’oranları tespit edildi.Bulgular: Nodüler guatr’lı erkek hastaların ortalama AgNOR sayısı (2,06 ± 0,46), kadınhastaların ortalama AgNOR sayısından (1,97 ± 0,49) anlamlı derecede yüksek değildi (p = 0,62).Erkek hastaların ortalama ‘‘TNA / ÇA’’ oranı (6,00 ± 1,11) ile Kadın hastaların ortalama ‘‘TNA/ ÇA’’oranı (5,34 ± 1,13) arasında da istatistiksel olarak anlamlı bir fark saptanmadı (p = 0,12).Buna ilaveten Kadın hastalarda ve erkek hastalarda hem ortalama AgNOR sayısı ile yaş (r =0,11 p = 0,56) hem de ‘‘TNA / ÇA’’oranı ile yaş arasında anlamlı bir korelasyon yoktu (r = 0,30;p = 0,11).Sonuç: Nodüler guatr tanılı hastaların tiroid hücrelerindeki AgNOR proteinlerinin sentez hızı,yaş veya cinsiyetten etkilenmemektedir. Bundan dolayı, bu proteinlerin hastalarda artışı hastalığabağlıdır

Anahtar Kelimeler:

AgNORs, NOR, nodüler guatr, tiroid ince iğne aspirasyon sitolojisi

Evaluation of “AgNOR Count / Nucleus” and “Total AgNOR Area / Nuclear Area’’ Proportions in Thyroide Cells of Cases with Nodular Goiter

Aim: NORs are loops of DNA, tandemly repeated that transcribed into ribosomal RNA (rRNA)processed into preribosomes in the nucleoli, and which eventually become part of matureribosomes in the cytoplasm. These centers are visible in interphase nuclei when examined aftera silver staining. The aim of this study was the comparison of the AgNOR count/nucleus and‘‘Total AgNOR area/ nuclear area’’ (TNA / NA) proportions to different age and sex in caseswith nodular goiter.Materials and Methods: Fifteen female (age range 38–68) and male (age range 38–68) whosefine needle aspiration biopsy (FNAB) were compatible with non-cystic nodular goiter wereincluded in the study. Those biopsy materials obtained by FNAB were stained for AgNORdetection according to a specific protocol. One hundred nuclei per individual have beenevaluated, and both mean AgNOR count and TNA / NA values of individual cells were detectedfor each cases.Results: Male patients with nodular goiter had not significantly higher mean AgNOR count(2.06 ± 0.46 %) than mean AgNOR count of females (1.97 ± 0.49) (p = 0.62). There is not anydifferences between mean TNA / NA ratio of males (6.00 ± 1.11) and females (5.34 ± 1.13) (p= 0.12). Additionally, there is not any correlation between age and mean AgNOR count (r =0.11 p = 0.56) and TNA / NA ratio (r = 0.30; p = 0.11) in both of females and males.Conclusion: The rate of AgNOR proteins synthesis is not effected by age or sex in thyroid cellsof cases with nodular goiter. Therefore, the increment of these proteins in patients is due todisease

Keywords:

NOR, AgNORs, nodular goiter, thyroid fine needle aspiration cytology,

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