Olgun KONTAŞ, Kemal DENİZ, Hatice KARAMAN, Ali MIZRAK, Salih Levent ÇINAR

GLUT-1, Ki-67 ve PCNA Antikorlarının Histopatolojik İmmünohistokimyasal Belirteçler Sedef Hastalığı ve Kronik Ayırıcı Tanı Süngeriyotik Dermatit

Giriş: Bu çalışma, bu çalışmanın faydalarını araştırmayı amaçlamaktadır. glukoz taşıyıcı 1'in (GLUT-1) immünohistokimyasal antikorları, nükleer protein Ki-67 ve çoğalan hücre nükleer antijeni (PCNA) sedef hastalığı ile kronik spongiotik dermatiti ayırt eder. Gereç ve Yöntem: 32 psoriazis vakasını ve 35 vakayı değerlendirdik. klinikopatolojik olarak teşhis edilmiş spongiotik dermatit. Deri redüksiyon mamoplasti prosedürlerinden elde edilen doku kontrol grubu olarak kullanıldı. H&E ile boyanmış cilt biyopsisi kesitleri incelendi. Ek olarak Ki-67, PCNA ve GLUT-1 dahil olmak üzere immünohistokimya yapıldı. Histolojik bulgular da kaydedildi Bulgular: Sedef hastalığı ile kronik arasında anlamlı bir fark yoktu. Ki-67 veya PCNA boyama sonuçlarında spongiotik dermatit grupları. GLUT1 dağılımı da benzerdi; ancak arada bir fark vardı. GLUT-1 yoğunluğu ile ilgili gruplar. Orta dereceli vakaların yüzdesi GLUT-1 boyaması psoriazis grubunda daha yüksekti, oysa yüzde Kronik spongiotik dermatit grubunda güçlü boyanma daha yüksekti. İncelenen histopatolojik özelliklerin çoğu sedef hastalığı ve kronik spongiotik dermatit olguları farklıydı. Sonuç: GLUT-1 boyamasının yoğunluğu uygun histopatolojik ve klinik bulguların sınırlı bir yararı olabilir. sedef hastalığı ve kronik spongiotik dermatitlerin ayırıcı tanısı.

The Use of GLUT-1, Ki-67 and PCNA Antibodies as Immunohistochemical Markers in Histopathological Differential Diagnosis of Psoriasis and Chronic Spongiotic Dermatitits

Introduction: The present study aims to investigate the benefits of the immunohistochemical antibodies of glucose transporter 1 (GLUT-1), the nuclear protein Ki-67, and proliferating cell nuclear antigen (PCNA) to distinguish between psoriasis and chronic spongiotic dermatitits. Materials and Methods: We evaluated 32 cases of psoriasis and 35 cases of spongiotic dermatitis that had been clinicopathologically diagnosed. Skin tissue from reduction mammoplasty procedures was used as a control group. Skin biopsy sections stained with H&E were examined. Additional immunohistochemistry was performed, including Ki-67, PCNA and GLUT-1. Histological findings were also noted Results: There was no significant difference between psoriasis and chronic spongiotic dermatitits groups in the results of Ki-67 or PCNA staining. GLUT1 distribution was also similar; however, there was a difference between the groups concerning GLUT-1 intensity. The percentage of cases with moderate GLUT-1 staining was higher in the psoriasis group, whereas the percentage of strong staining was higher in the chronic spongiotic dermatitits group. Most of the examined histopathological features of psoriasis and chronic spongiotic dermatitits cases were different. Conclusion: The intensity of GLUT-1 staining with the appropriate histopathological and clinical findings may have a limited benefit in the differential diagnosis of psoriasis and chronic spongiotic dermatitits.

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