Aslı Aybike DOĞAN, Şeyma TAŞDEMİR, Aylin Şendemir ÜRKMEZ

SH-SY5Y VE SK-N-AS HÜCRE HATLARININ MİKRODOKU OLUŞTURMA KAPASİTELERİNİN KARŞILAŞTIRILMASI

İki boyutlu (2B) hücre kültür sistemleri temel in vitro araştırmalar için önemli araçlar olmasına rağmen, biyokimyasal sinyal iletimi, hücre-hücre ve hücre-matris etkileşimleri gibi kompleks koşulları taklit ederken, in vivo modellere göre zayıf kalmaktadır. Bu çalışmada, in vitro nörodejeneratif hastalık model hücreleri olarak sıklıkla kullanılan SH-SY5Y ve SK-N-AS nöroblastom hücre hatlarının, 3D Petri Dish® tekniği ile mikrodoku oluşturma kapasiteleri karşılaştırılmış olup, mikrodokuların morfolojileri gözlemlenmiş ve boyut analizleri gerçekleştirilmiştir. Sonuç olarak, SHSY5Y mikrodokularının nörodejeneratif hastalıklar ve nöroblastoma çalışmaları için alternatif bir 3B model olabileceği belirlenmiştir

Anahtar Kelimeler:

Hücre kültürü, Üç-boyutlu (3B) hücre kültürü, Mikrodoku, SH-SY5Y, SK-N-AS

COMPARISON OF MICROTISSUE FORMING CAPACITY OF SH-SY5Y AND SK-N-AS CELL LINES

Two-dimensional (2D) cell culture systems are important tools for basic in vitro research. However, they form a thin monolayer structure and poorly mimic the complex in vivo conditions in terms of biochemical signals, cell-cell and cell- extracellular matrix (ECM) interactions. In this study, we performed a comparative study on SH-SY5Y and SK-N-AS neuroblastoma cell lines in terms of their microtissue forming capacity. Both cell lines are commonly used to model neurodegenerative diseases in vitro. Cells were cultured using 3D Petri Dish technique. The cells’ microtissue forming capacity was observed morphologically and microtissues’ size was analized. Results indicate that microtissue forming capacity of SH-SY5Ycell line was better than that of SK-N-AS cell line. SH-SY5Y microtissues can be used as an alternative, scaffold-free in vitro 3D model for neurodegenerative diseases and neuroblastoma research

Keywords:

Cell culture, Three-dimensional (3D) Cell culture, Microtissue, SH-SY5Y, SK-N-A,

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