Reyhan Selin UYSAL, SERDAL SABANCI, ÖZLEM AKPINAR, Ufuk BÖLÜKBAŞI, Levent YILMAZ

XYLITOL BIOPRODUCTION FROM TOBACCO STALK

Bu çalışmanın amacı tütün sapından ksiloz eldesi ve ksilozun Candida tropicalis ile ksilitole dönüşümüdür. Ksilitol, beş karbonlu şeker alkolüdür. Ksilozu ekstrakte etmek için tütün sapı seyreltik sülfirik asit ile hidroliz edilmiştir. Hidrolizat Candida tropicalis ile fermente edilmiştir. Yüksek verimlilikte ksilitol üretimi için hidrolizat, oksijen ve inokülüm konsantrasyonu optimize edilmiştir. Optimum koşullar altındahidrolizat %59 verimlilikte ve 0.18 g/l-sa volümetrik üretim hızında ksilitole fermente olmuştur.

(TÜTÜN SAPINDAN KSİLİTOLÜN BİYOÜRETİMİ)

The aim of this study was to produce xylose from tobacco stalk and conversion of xylose to xylitol production by Candida tropicalis. Xylitol is a five-carbon sugar alcohol. Tobacco stalk was hydrolyzed with dilute sulphuric acid to extract xylose. Fermentation ofhydrolysate was performed by Candida tropicalis. The hydrolysate, oxygen and inoculum concentration were optimized for the production of xylitol with high yield. Under the optimum conditions, the hydrolysate was fermented to xylitol with 59% yield and 0.18 g/l-hr volumetric production rate.

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