Neslihan DEMİRCİ, Çiğdem DEMİRKAYA, Zeliha DEMİREL, Esra İMAMOĞLU, Meltem Conk DALAY

MALİK ASİT KONSANTRASYONUNUN EKSTREMOFİLİK KIRMIZI MİKROALG GALDERİA SULPHURARİA DA ARAŞTIRILMASI

Malik asit yaşayan organizmaların ürettiği ve malatlar gibi malik asit tuzları olduğu bilinen bir dikarboksilik asittir. Karbohidrat, yağ ve protein metabolizmasını birleştiren pürivat/malat döngüsünü içinde malatlar kullanılır. Bu döngü temelinde, malik asit Galderia sulphuraria nın karbonhidrat oranını değiştirebildiğini tahmin ediyoruz. Ayrıca bu konudaki benzer çalışmalara rastlanamamıştır. Bu çalışmada, malik asit konsantrasyonlarının ekstremofilik kırmızı alg G. Sulphuraria (SAG 108.79) büyümesi (maksimum spesifik büyüme oranı ve ikileneme süresi) üzerine etkisi incelenmiştir. Artan malik asit konsantrasyonunda spesifik büyüme oranı ve biykütle konsantrasyonu artarken, 5 mM konsantrasyonda proteinin en yüksek konsantrasyonu (153,81 ± 0,673 mg / L) belirlenmiştir. Sonuçlarımız karbonhidrat miktarında yaklaşık % 8,35 artış olduğunu göstermektedir

Anahtar Kelimeler:

Malik asit, Yağ, Protein, Karbonhidrat, Galderia sulphuraria, Mikroalg

INVESTIGATION OF THE MALIC ACID CONCENTRATION ON EXTREMOPHILIC RED MICROALGA GALDIERIA SULPHURARIA

Malic acid is a dicarboxylic acid which is made by living organisms and the salts of malic acid are known as malates. Malates are used in pruvate/malate cycle that merges carbohydrate, fat and protein metabolism. Based on this cycle, the prediction was that malic acid could change carbohydrates ratio in G.sulphuraria. In this study, the effects of malic acid concentrations were investigated on growth (maximum specific growth rate and doubling time) of extremophilic, red alga Galdieria sulphuraria (SAG 108.79). By increasing malic acid concentration, specific growth rate and biomass concentration were also increased, however the highest concentration of protein (153.81±0.673 mg/L) was obtained in 5 mM. The total amount of carbohydrates was increased about 8.35%

Keywords:

Malic acid, Lipid, Protein, Carbohydrate, Galdieria sulphuraria, Microalga,

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