Pınar TALAY PINAR, Tamer TULUK, Zühre ŞENTÜRK

Pravastatin’in Camımsı Karbon Elektrot Üzerinde Karedalga Voltametrisi ile İlaç Formülasyonunda Tayini

Lipit düşürücü ilaç olan Pravastatin′in, camımsı karbon elektrot üzerindeki elektrokimyasal davranışı, Britton-Robinson tamponu içerisinde dönüşümlü voltametri ve kare-dalga voltametrisi teknikleri kullanılarak araştırılmıştır. Dönüşümlü voltametrik çalışmalar, molekülün tersinmez olarak yürüyen yükseltgenmesinin difüzyon-kontrollü olduğunu göstermiştir. Kare-dalga voltametrisi kullanılarak, Pravastatin′in pH 2.0 değerinde +1.24 V (vs. Ag/AgCl) gerilim değerinde 2.4×10-7-2.8×10-6 M derişim aralığında (saptama sınırı, 5.0×10-8 M) tayini gerçekleştirilmiştir. Önerilen yöntem, Pravastatin′in tablet ilaç şeklinden tayinine başarıyla uygulanmıştır.

Determination of Pravastatin Drug Formulation by Squarewave Voltammetry on Glassy Carbon Elektrode

The electrochemical behavior of Pravastatin, a lipid-lowering drug, on a glassy carbon electrode wasexplored in Britton-Robinson buffer by using cyclic and square-wave voltammetry. Cyclicvoltammetric studies indicated that the compound underwent irreversible oxidation, which was adiffusion-controlled process. Using square-wave voltammetry, Pravastatin could be determined at+1.24 V (vs. Ag/AgCl) in the concentration range 2.4×10-7-2.8×10-6 M at pH 2.0, with a detectionlimit of 5.0×10-8 M. The suggested method was successfully applied for the assay of Pravastatin intablets.

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[1] Al-Badr, A.A., Mostafa, G.A.E. 2014. Pravastatin Sodium, Profiles Drug Subst. Excip. Relat. Methodol., vol. 39, p. 433-513.
[2] Endo, A., Kuroda, M., Tanzawa, K. 2004. Competitive Inhibition of 3-hydroxy-3-methyl glutaryl Coenzyme A Reductase by ML-236A and ML-236B Fungal Metabolites, Having Hypocholesterolemic Activity, Atheroscler Suppl., vol. 5, p. 39-42.
[3] Brown, M.S., Faust, J.R., Goldstein, J.L., Kaneko, I., Endo, A. 1978. Induction of 3-hydroxy-3- methylglutaryl Coenzyme A Reductase Activity in Human Fibroblasts Incubated with Compacti n (ML236B), A Competitive Inhibitor of The Reductase., J. Biol Chem., vol. 253-4, p. 1121- 1128.
[4] Manzoni, M., Rollini, M., Biosynthesis and Biotechnological Production of Statins by Filamentous Fungi and Application of These Cholesterol-Lowering Drugs., Appl Microbiol Biotechnol., vol. 58, p. 555-564.
[5] Sorrentino, M.J. 2012. An Update on Statin Alternatives and Adjuncts, Clin. Lipidol., vol. 7, p. 721-730.
[6] Antal, I., Koneracka, M., Zavisova, V., Kubovcikova, M., Kormosh, Z., Kopcansky, P., Statins Determination: A Review of Electrochemical Techniques, Crit. Rev. Anal. Chem. Vol. 47-6, p. 474- 489.
[7] Siekmeier, R., Gross, W., März, W. 2000. Determination of Pravastatin by High Performance Liquid Chromatography, Int. J. Clin. Pharmacol. Ther., vol. 38-9, p. 419-25.
[8] Ashour, S., Nakshbandi, H., Omar, S. 2008. Quantitative Determination of Pravastatin in Pharmaceutical Dosage Forms by High-Performance Liquid Chromatography with Ultraviolet Detection., Int. J. Biomed.Sci., vol. 4-2, p. 135-139.
[9] Silva, T.D., Oliveira, M.A., de Oliveira, R.B., ViannaSoares, C.D., Development and Validation of a Simple and Fast HPLC Method for Determination of Lovastatin, Pravastatin and Simvastatin., J Chromatogr Sci., vol. 50-9, p. 831-838.
[10] Athota, R.V., Jagarlapudi, S.K., Singampalli, M.R. 2017. Stability Indicating HPLC Method for the Simultaneous Quantification of Aspirin and Pravastatin in bulk and Tablets: Method Development and Validation., J. Appl. Pharm. Sci., vol. 70-3, p. 48-56.
[11] Balaji, S., Suman, Katteboina. 2009. Development of Spectrophotometric Method for Determination of Pravastatin Sodium in Bulk and Tablet Formulations, Int. J. Pharm.Tech. Res., vol. 1-4 , p. 1017-1019.
[12] El-Olemy, A. 2017. Simultaneous UV Spectrophotometric Determination of Pravastatin Sodium and Pioglitazone Hydrochloride in Pharmaceutical Preparations, J.A.P.R., vol. 1-3 p. 143-149.
[13] Kawabata, K., Samata, N., Urasaki, Y., Quantitative Determination of Pravastatin and R-416, Its Main Metabolite in Human Plasma, by Liquid Chromatography-Tandem Mass Spectrometry., J Chromatogr B Analyt Technol Biomed Life Sci., vol. 816-(1-2), p. 73-79.
[14] Önal, A., Sagirli, O. 2006. Development of a Selective LC Method for the Determination of Pravastatin Sodium, Chromatographia, vol. 64- (3-4), p. 1-6.
[15] Chen, L., Joshi, P., Piatkivskyi, A., Aguilar, K., Lin, J. 2017. Method Development and Validation for the Determination of Pravastatin in Human Plasma by LC-MS/MS, J. Bioanal. Biomed., vol. 9-3, p. 137-143.
[16] Üstün, Z., Daldal, Y.D., Aydoğan, A.T., Çubuk Demiralay, E. 2017. An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by Using Reversed Phase Liquid Chromatography Method, Karaelmas Sci. Eng. J., vol. 7-2, p. 602-607.
[17] Kırcalı, K., Tunçel, M., Aboul-Enein, H.Y. 2004. Determination of Pravastatin in Tablets by Capillary Electrophoresis, Il Farmaco, vol. 59-3, p. 241-244.
[18] Nigoviç, B., Vegar, I. 2008. Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products, Croat. Chem. Acta, vol. 81-4, p. 615-622.
[19] Talay Pınar, P., Şentürk, Z. 2017. Voltammetric Investigation of Antiviral Drug Valacyclovir at a Boron-Doped Diamond Electrode in Different Electrolyte Media: Its Determination Enhanced by Anionic Surfactant in Pharmaceuticals and Biological Fluids, Curr. Pharm. Anal., vol. 13-2, p. 175-187.
[20] Alpar, N., Talay Pınar, P., Yardım, Y., Şentürk, Z. 2017. Voltammetric Method for the Simultaneous Determination of Melatonin and Pyridoxine in Dietary Supplements Using a Cathodically Pretreated Boron-doped Diamond Electrode, Electroanalysis, vol. 29-7, p. 1691-1699.
[21] Talay Pınar, P., Yardım, Y., Şentürk, Z. 2018. Electrochemical Oxidation of Ranitidine at Poly(dopamine) Modified Carbon Paste Electrode: Its Voltammetric Determination in Pharmaceutical and Biological Samples Based on the Enhancement Effect of Anionic Surfactant, Sens. Actuat. B: Chem., vol. 273, p. 1463-1473.
[22] Coşkun, N.Y., Aycan, S., Sungur, S. 1997. Differential Pulse Polarographic Determination of Pravastatin Sodium in Tablets, Die Pharmazie, vol. 52, p. 485- 486.
[23] Nigovic, B. 2006. Electrochemical Properties and Square-Wave Voltammetric Determination of Pravastatin, Anal. Bioanal. Chem., vol. 384, p. 431- 437.
[24] Neves, M.M.P.S., Nouws, H.P.A., Delerue-Matos, C. 2010. Carbon Surfaces for the Oxidative Quantification of Pravastatin: Glassy Carbon vs. Screen-Printed Carbon Electrodes, J. Food Drug Anal., vol. 18-5, p. 353-357.
[25] Bard, A.J., Faulkner, L.R. 2001. Electrochemical Methods: Fundamentals and Applications, 2nd ed.; John Wiley & Sons: New York.
[26] Ishihama, Y., Nakamura, M., Miwa, T., Kajima, T., Asakawa, T. 2002. A Rapid Method for pKa Determination of Drugs Using Pressure-Assisted Capillary Electrophoresis with Photodiode Detection in Drug Discovery, J. Pharm. Sci., vol. 91, p. 933-942.