Yağmur HAMURCİ, Murat IHLAMUR, Yağmur ZENGİN

Elettaria Cardamomum Ekstraktının Proleukin İlacı Kombinasyonu ile Mide Kanseri Hücre Hattı Üzerindeki İmmünostimulan/Sitotoksik Etkilerinin İncelenmesi

Kanser, hücrelerin kontrolsüz çoğalması sonucunda meydana gelmektedir. Farklı kanser türleri olmakla birlikte mide kanseri dünyada en çok görülen kanser türleri arasında yer almaktadır. Mide kanserine karşı birçok tedavi yaklaşımı geliştirilmiştir. Ancak günümüzde kullanılan tedavi yöntemleri, kanser hücrelerini öldürmekle birlikte sağlıklı hücrelere de zarar vermektedir. Kullanılan tedavi yöntemlerinin dezavantajlarından dolayı bitkisel tedavi yaklaşımları kullanılmaya başlanmıştır. Elettaria Cardamomum (E. Cardamomum) (kakule) ekstraktında bulunun Diindolilmetan (DIM) ve indol-3-karbinol (I3C) molekülleri hormon yolaklarını baskılayarak kanser hücrelerini öldürdüğü literatürde belirtilmektedir. Bu çalışmada, E. Cardamomum ve proleukin ilaç kombinasyonlarının AGS, J774, THP-1 hücre hatları üzerindeki etkisi araştırılmıştır. Sonuç olarak, kakule ekstraktında bulunan I3C ve DIM molekülleri sayesinde AGS mide kanseri hücre hattında yüksek sitotoksik etkiye sahip olduğu düşünülmektedir. Kakule ekstraktı ile kombine edilen proleukin ilacının ise AGS hücrelerinde kakule ekstraktının tek başına kullanımına göre daha fazla öldürme oranına sahip olduğu belirlenmiştir.

Anahtar Kelimeler:

Mide kanseri, AGS, E. Cardamomum, Sitotoksisite

Investigation of Immunostimulant/Cytotoxic Effects of Elettaria Cardamomum Extract with Combination of Proleukin Drug on Gastric Cancer Cell Line

Cancer occurs as a result of uncontrolled proliferation of cells. Although there are different types of cancer, gastric cancer is among the most common cancer types in the world. Many treatment approaches have been developed against gastric cancer. However, the treatment methods used today not only kill cancer cells but also damage healthy cells. Due to the disadvantages of the treatment methods used, herbal treatment approaches have started to be used. It is stated in the literature that Diindolylmethane (DIM) and indole-3-carbinol (I3C) molecules in Elettaria Cardamomum (E. Cardamomum) extract kill cancer cells by suppressing hormone pathways. In this study, the effect of E. Cardamomum and proleukin drug combinations on AGS, J774, THP-1 cell lines was investigated. As a result, it was determined that cardamom extract has a high cytotoxic effect on AGS gastric cancer cell line thanks to I3C and DIM molecules. It was determined that proleukin drug combined with cardamom extract had a higher rate of killing in AGS cells than the use of cardamom extract alone.

Keywords:

Gastric cancer, AGS, E. Cardamomum, Cytotoxicity,

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