Mustafa ATEŞ, M. Hakan KÖKSAL, M. Fevzi CELAYİR, Adil BAYKAN

Sıçanlarda intestinal derin iskemi-reperfüzyon modelinde ginkgo biloba ekstresinin (EGb761) profi laksi ve tedavide kullanımının mortalite üzerine etkisi

Amaç: Ginkgo Biloba ekstresi (EGb761) yaklasık 5000 yıldır Çinliler tarafından kullanılan bir bitki olan Ginkgo Biloba yapraklarının standardize edilmis sekli olup antioksidan etkinliği bilinmektedir. Bu çalısmada Megison’un sıçanlarda gelistirdiği intestinal “derin iskemi” modelinde EGb761 kullanımının mortalite üzerine olan etkisinin arastırılması amaçlandı. Gereç ve Yöntemler: Çalısmada 138 adet erkek SpraqueDawley cinsi sıçan kullanıldı. Sıçanlar 4 gruba ayrıldı; 1.Grup (Kontrol grubu), II. Grup (Derin iskemi-reperfüzyon grubu), III. Grup (Derin iskemi-reperfüzyon-EGb 761 profi laksi ve tedavi grubu), IV. Grup (Derin iskemi esnasında EGb 761 ile tedavinin yapıldığı grup). Derin iskemi 30 dakika olarak uygulandı. İslem sonrası ilaç uygulamaları ve sıçan takibi 1 hafta yapıldı. Gruplarda bir haftalık takip süresinde olusan mortalite sayıları karsılastırıldı. Bulgular: I.grupta 2 (%7.7), II. grupta 22 (%61.1), III. Grupta 6 (%13.6), IV. grupta 11 (%34.3) sıçan laparotomi sonrası bir haftalık takip sürecinde mortalite olustu. EGb761’nın profi laksi ve tedavi amacı ile kullanımının derin iskemi modelinde mortaliteyi istatistiksel olarak anlamlı oranda azalttığı görüldü (p

Prophylactic and therapeutic effect of ginko biloba extract (Egb761) on mortality of intestinal deep ischemia-reperfusion model in rats

Objectives: Ginkgo biloba extract (EGb761) is a standardized form of Ginkgo Biloba plant leaves which have been used by Chines nearly 5000 years and Its’ antioxidant activity is known. In this study we aimed to investigate effect use of EGb761 on mortality in Megison’un deep ischemia reperfusion model of rats. Materials and Methods: 138 male Spraque-Dawley rats were used in this study. The rats were divided into 4 groups: Group I (control group), Group II (deep ischemiareperfusion group), Group III (Group of prophylaxis and treatment of deep ischemia-reperfusion with EGb 761), Group IV (group of treatment with EGb 761 during deep ischemia). Deep ischemia was applied 30 minutes. Rats were followed-up one week after laparotomy. Differences between numbers of mortality in groups during one week follow-up were compared. Results: Number of died rats in Group I, II, III, and IV during one week follow-up were 2 (7.7%), 22 (61.1%), 6 (13.6%), and 11 (34.3%) respectively. Mortality rate decreased statistically signifi cant with use of EGb761 with prophylactic and therapeutic purposes (p<0.001, p<0.028). Conclusion: EGb761’s prophylactic and therapeutic benefi t on intestinal ischemia reperfusion injury was observed. However, these results should be supported with further biochemical and histopathological studies.

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